GenWay/Sphingosine kinase 2, Active Human Recombinant Protein/GWBF95010/20µg

Description:

Description:HumanSphingosinekinase2(GenBankAccessionNo.AF245447),fulllengthwithN-terminalHistag,MW=69.5kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.SpecificActivity:20U/µg.SphingosinekinaseactivitywasdeterminedusingDerythro-sphingosineandATPassubstrates.Aunitofsphingosinekinaseactivityisdefinedastheamountofenzymerequiredtoproduce1pmolofS1P/min.Assaybuffer:50mMHEPES,pH7.4,150mMNaCl,5mMMgCl2,1mMDTT,3µMNaorthovanadate,0.5mMATP,4µMDerythro-sphingosine,and0.75µg/mlsphingosinekinase2.Application:Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.Stability:>6monthsat-80°C.References:1.Zemann,B.etal.,Blood.107(4):1454-8(2006)2.Maceyka,M.etal.,JBiolChem.280(44):37118-29(2005)

Host:baculovirus

Tag:N-terminalHis-tag

GINum:AF245447

MW:69.5kDa

Format:aqueousbuffersolution

Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole

ScientificCategory:Kinase/Lipids

Species:human

AdditionalInformation:

Name	Sphingosinekinase2,ActiveHumanRecombinantProtein
RelatedProductNames	Sphingosinekinase2,ActiveHumanRecombinantProtein
MolecularWeight	69.5kDa
Purity	40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Source	baculovirus
GINumber	AF245447
Format	AqueousBufferSolution
Reactivity	Human
Storage	-80°C
IntendedUse	ResearchUseOnly