GenWay/Caspase9 Fluorometric Assay Kit 25 assays/GWBAXR058/25 assays

Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-9FluorometricAssayKitprovidesasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceLEHD.TheassayisbasedondetectionofcleavageofsubstrateLEHD-AFC(AFC:7-amino-4-trifluoromethylcoumarin).LEHD-AFCemitsbluelight(λmax=400nm);uponcleavageofthesubstratebycaspase-9orrelatedcaspases,freeAFCemitsayellow-greenfluorescence(λmax=505nm),whichcanbequantifiedusingafluorometerorfluorecencemicrotiterplatereader.
Summary:
-Detectionmethod-Flowcytometry(400nmexcitationfilterand505nmemissionfilter),fluorecencemicrotiterplatereaderandfluorescencemicroscopy
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-LEHD-AFC(1mM)
-DTT(1M)
FeaturesandBenefits:
-Simpleone-stepprocedure;takes1-2hours
-Fastandconvenient
-ComparisonofthefluorescenceofAFCfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-9activity.
ShippingConditions:Gelpack

AdditionalInformation:

Name	Caspase-9FluorometricAssayKit-25assays
RelatedProductNames	Caspase-9FluorometricAssayKit,Caspase-9,capase9,caspase9,Caspase-9Substrate,LEHD,LEHD-AFC,Caspase-9assay,Caspase-9FluorometricAssay,LEHDase
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly