GenWay/Caspase9 Colorimetric Assay Kit 100 assays/GWBAXR060/100 assays

Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-9ColorimetricAssayKitprovidesasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceLEHD.Theassayisbasedonspectrophotometricdetectionofthechromophorep-nitroanilide(pNA)aftercleavagefromthelabeledsubstrateLEHD-pNA.ThepNAlightemissioncanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat400-or405nm.
Summary:
-Detectionmethod-Absorbance(400or405nm)
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-LEHD-pNA(4mM)
-DTT(1M)
-DilutionBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takes1-2hours
-Fastandconvenient
-ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseinCaspase-9activity.
ShippingConditions:Gelpack

AdditionalInformation:

Name	Caspase-9ColorimetricAssayKit-100assays
RelatedProductNames	Caspase-9ColorimetricAssayKit,Caspase-9,capase9,caspase9,Caspase-9Substrate,LEHD,LEHD-pNA,Caspase-9assay,Caspase-9ColorimetricAssay,LEHDase
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly