GenWay/Fluorogenic HDAC class 2a substrate/GWB2F8206/250nmole

Description:

Description:Afluorogenic,acetylatedpeptidesubstrateforHDAC4,5,7and9.PURITY:>95%byHPLC.APPLICATIONS:Studyenzymekinetics,andscreensmallmoleculeinhibitorsofHDACclass2afordrugdiscoveryandHTSapplications.SUPPLIEDAS:DMSOsolutionQUANTITY:50µl@5mMSTORAGE:-20°Cor-70°C.AssayProtocol:Material:Assaybuffer,BPScatalognumber50031;Assaydeveloper,BPScatalognumber50030;HDACSubstrate,catalognumber50040,Step1:AddingallreactionmixturetoalowbindingNUNCblackplate,VWRcatalognumber62408-936,5ulofHDAC4,0.01ug/ul,35ulofHDACassaybuffer,BPScatalognumber50031,5ulof1mg/mlBSA5ulof200uMsubstrate,BPScatalognumber50040,Incubateat37°Cfor30min.Step2:StopthereactionAdd50ulofHDACassaydeveloper,2x,BPScatalognumber50030,andincubatetheplateatroomtemperaturefor15minStep3:Readsampleinamicrotiter-platereadingfluorimetercapableofexcitationatawavelengthintherange350-380nmanddetectionofemittedlightintherange440-460nm.

Format:DMSOsolution

Formula:5mMsolutioninDMSO

ScientificCategory:Deacetylase/Substrate

AdditionalInformation:

Name	FluorogenicHDACclass2asubstrate
RelatedProductNames	histonedeacetylase,HDAC
Purity	5mMsolutioninDMSO
Format	DmsoSolution
Storage	-20°Cor-80°C
IntendedUse	ResearchUseOnly