GenWay/Caspase10 Colorimetric Assay Kit 400 assays/GWBAXR081/400 assays

Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-10ColorimetricAssayKitprovidesasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceAEVD.Theassayisbasedonspectrophotometricdetectionofthechromophorep-nitroanilide(pNA)aftercleavagefromthelabeledsubstrateAEVD-pNA.ThepNAlightemissioncanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat400-or405nm.
Summary:
-Detectionmethod-Absorbance(400or405nm)
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-AEVD-pNA(4mM)
-DTT(1M)
-DilutionBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takesonly1-2hours
-Fastandconvenient
-ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseinAEVD-dependentCaspaseactivity.
ShippingConditions:Gelpack

AdditionalInformation:

Name	Caspase-10ColorimetricAssayKit-400assays
RelatedProductNames	Caspase-10ColorimetricAssayKit,Caspase-10,caspase10,caspase10,Caspase-10Substrate,AVED,AEVD-pNA,Caspase-10assay,Caspase-10ColorimetricAssay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly