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GenWay/Chicken IgY ELISA/GWB374Z14/96 well

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NameChickenIgYELISA
RelatedProductNamesIgY,ImmunoglobulinY,ChickenIgY,ChickenImmunoglobulinY,ChickenImmunoglobulinYELISA
AliasSymbolsFCR
ProteinNameIgY
SpeciesReactivityChicken
AssayInfoAssayMethodology:QuantitativeSandwichELISA
Sensitivity1.508ng/ml
KitRange6.25ng/ml-400ng/ml
HomologyChicken
StandardRange400ng/ml-6.25ng/ml
SampleTypeSerum,plasmaandotherbiologicalfluids
ProductFormatInSolution
Specimen/VolumesReferToManual
ConcentrationLotSpecific
ApplicationsELISA
ReconstitutionandStorage1.DILUENT
The5XDiluentConcentrateisstableuntiltheexpira-tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionsshouldbestoredat4-8C.
2.WASHSOLUTION
The20XWashSolutionConcentrateisstableuntiltheexpira¬tiondate.The1Xworkingsolutionisstableforatleastoneweekfromthedateofpreparation.Bothsolutionscanbestoredatroomtemperature(1625C)orat4-8C.
3.ENZYME-ANTIBODYCONJUGATE
Undilutedhorseradishperoxidaseanti-IgYconjugateshouldbestoredat4-8Canddilutedimmediatelypriortouse.Theworkingconjugatesolutionisstableforupto8hours.
4.CHROMOGEN-SUBSTRATESOLUTION
TheSubstrateSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
5.STOPSOLUTION
TheStopSolutionshouldbestoredat4-8Candisstableuntiltheexpirationdate.
6.ANTI-CHICKENIgYELISAMICROPLATE
Anti-ChickenIgYcoatedwellsarestableuntiltheexpirationdate,andshouldbestoredat4-8Cinsealedfoilpouchwithdesiccantpack.
7.CHICKENIgYCALIBRATOR
TheChickenIgYCalibratorshouldbestoredat4C.Theworkingstandardsolutionsshouldbepreparedimmediatelypriortouseandarestableforupto8hours.
ReactivityChicken
KitSize96well
KitPrincipleGenwayBiotechIgYELISAKit(Chicken)(GWB-374Z14)isbasedonstandardsandwichenzyme-linkedimmuno-sorbentassaytechnology.AnantibodyforIgYhasbeenpre-coatedontoa96-wellplate(12x8WellStrips).Standardsortestsamplesareaddedtothewells,incubatedandwashedtoremoveunboundproteins.AnAnti-IgY-HRPconjugateddetectorantibodyisthenadded,incubatedandunboundconjugateiswashedaway.AnenzymaticreactionisproducedthroughtheadditionofTMBsubstratewhichiscatalyzedbyHRPgeneratingabluecolorproductthatchangestoyellowafteraddingacidicstopsolution.Thedensityofyellowcolorationreadbyabsorbanceat450nmisquantitativelyproportionaltotheamountofsampleIgYcapturedinthewell.
KitComponent
ComponentAmount
Anti-ChickenIgYMicroplate96Wells(12x8WellStrips)
20XDiluentConcentrate50mL
20XWashSolution50mL
100XEnzyme-AntibodyConjugate150uL
Chromogen-SubstrateSolution12mL
StopSolution12mL
ChickenIgYCalibrator1
KitDuration70Minutes
StorageRefertokitinstructionsforcomponentstorage
LeadTimeDomestic:within1weekdelivery International:1week
IntendedUseResearchUseOnly
AdditionalInformationIntendedUse:ThetotalChickenIgYtestkitsareahighlysensitivetwo-siteenzymelinkedimmunoassay(ELISA)formeasuringIgYinserumorplasmaofChickens.
::Reagents:
1.DILUENTCONCENTRATE(RunningBuffer)
Onebottlecontaining50mlofa5Xconcentrateddiluentrunningbuffer.
2.WASHSOLUTIONCONCENTRATE
Onebottlecontaining50mlofa20Xconcentratedwashsolution.
3.ENZYME-ANTIBODYCONJUGATE100X
Onevialcontaining150ulofaffinitypurifiedanti-ChickenIgYantibodyconjugatedwithhorseradishperoxidaseinastabilizingbuffer.
4.CHROMOGEN-SUBSTRATESOLUTION
Onevialcontaining12mLof3,3’,5,5’-tetramethybenzidine(TMB)andhydrogenperoxideincitricacidbufferatpH3.3.
5.STOPSOLUTION
Onevialcontaining12ml0.3Msulfuricacid.
WARNING:Avoidcontactwithskin.
6.ANTI-CHICKENIgYELISAMICROPLATE
Twelveremovableeight(8)wellmicrowellstripsinwellholderframe.Eachwelliscoatedwithaffinitypurifiedanti-ChickenIgY.
7.CHICKENIgYCALIBRATOR
OnevialcontainingChickenIgYcalibrator.