GenWay/ACK1, Active Human Recombinant Protein/GWBD2EC8D/10µg

Description:

Description:HumanAck1(GenBankAccessionNo.NM_005781),a-a110-476(end)withN-terminalGSTtag,MW=68kDa,expressedinSf9insectcellsystems.SpecificActivity:88pmol/min/mg.Assayconditions:Add50µlreactionmix(ACK1dilutedin1xkinasebuffersupplementedwith0.2μMATP)towellscoatedwithsubstrate.Incubatefor1h.AddEuropium-labeledantip-Tyrantibodyandincubatefor1h.Timeresolvedfluorescencemeasuredatroomtemperatureat?exc=330+/-40nmand?em=620+/-10nm.Application:Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.Stability:>6months

Host:baculovirus

Tag:N-terminalGSTtag

GINum:NM_005781

MW:68kDa

Format:aqueousbuffersolution

Formula:40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione

ScientificCategory:Kinase/CytoplasmicTyr

Species:human

AdditionalInformation:

Name	ACK1,ActiveHumanRecombinantProtein
RelatedProductNames	ACK1,ActiveHumanRecombinantProtein
MolecularWeight	68kDa
Purity	40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione
Source	baculovirus
GINumber	NM_005781
Format	AqueousBufferSolution
Reactivity	Human
Storage	-80°C
IntendedUse	ResearchUseOnly