GenWay/Nuclear/Cytosol Fractionation Kit 100 assays/GWBAXR179/100 assays

Description:

Description:ThisNuclear/CytosolExtractionKitprovidesacompletesystemthatenablestheseparationofnuclearextractfromthecytoplasmicfractionofmammaliancells.Theoptimizedreagentsandproceduresprovidedwiththekitallowseparationofnuclearandcytoplasmicfractionsquicklywithlittleornocross-contaminations.Theextractednuclearandcytoplasmicproteinfractionsarefunctionalandcompatiblewithdownstreamassayssuchastranscriptionalactivity,RNAsplicing,gelshiftassay,reporterassays,enzymeactivityassays,andWesternblotting.
Summary:
-Sampletype-Cellculture(adherentandsuspension),freshandfrozentissues
-Speciesreactivity-Mammalian
-Kitsize-Conveientsizes(25,100)
-Applications-ThisNuclear/CytosolExtractionKitprovidesacompletesystemthatenablestheseparationofnuclearextractfromthecytoplasmicfractionofmammaliancellsandtissuesThenewkitprovidesasystemthatmaintainsthenuclearandcytoplasmiccompartmentsseparateandintact.Transcriptionalactivity/reporterassays/enzymeactivityassays/Westernblottingandmoredownstreamassays.
Kitcomponents:
-CytosolExtractionBufferA(CEB-A)
-CytosolExtractionBufferB(CEB-B)
-NuclearExtractionBuffer(NEB)
-DTT(1M)
-ProteaseInhibitorCocktail(lyophilized)
FeaturesandBenefits:
-Simpleprocedure;takeslessthan2-3hours
-Fastandconvenient
-Theoptimizedreagentsandproceduresprovidedwiththekitallowseparationofnuclearandcytoplasmicextractsfromcellsrelativelyquickwithlittleornocross-contaminations
ShippingConditions:Gelpack

AdditionalInformation:

Name	Nuclear/CytosolFractionationKit-100assays
RelatedProductNames	Nuclear/CytosolFractionationKit,nuclearfractionantionkit,cytosolfractionationkit,nuclearisoaltion,cytosolisolation
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly