GenWay/Mouse TNF alpha ELISA Kit/GWBZZD049/1x96 well plate

Description:

Tumornecrosisfactor-alpha(TNF-alpha,orTNF)issecretedbymacrophagesinresponsetoinflammation,infectionandcancer.HumanTumorNecrosisFactor(TNF)andLymphotoxin(TNF-beta)arecytotoxicproteinswhichhavesimilarbiologicalactivitiesandshare30%aminoacidhomology.TNF-alphaisproducedbymonocytes,whichcanstimulateendothelialcellstoproducethemultilineagegrowthfactorgranulocyte-macrophagecolony-stimulatingfactorandextendtheroleofthisimmunoregulatoryproteintotheregulationofhematopoiesisinvitro.TNFisasolubleproteinthatcausesdamagetotumorcellsbuthasnoeffectonnormalcells.HumanTNFhasbeenpurifiedtoapparenthomogeneityasa17.3-kilodaltonproteinfromHL-60leukemiacellsandhasshowedcytotoxicandcytostaticactivitiesagainstvarioushumantumorcelllines.ThehumanTNFcDNAis1585basepairsinlengthandencodesaproteinof233aminoacids.Thematureproteinbeginsatresidue77,leavingalongleadersequenceof76aminoacids.TNF-alphahasbeenmappedtohumanchromosome6.

AdditionalInformation:

Name	MouseTNFalphaELISAKit
RelatedProductNames	APC1protein|Cachectin|DIF|solubleform|Tnf|TNFa|TNFalpha|TNFmacrophagederived|TNFmonocytederived|TNFsuperfamilymember2|TNF-a|TNF-alpha|TNFA_HUMAN|TNFSF2|Tumornecrosisfactor|Tumornecrosisfactoralpha|Tumornecrosisfactorli
ShortDescription	SandwichELISAkitofQuantitativeDetectionforMouseTNFalpha
Immunogen	pleaseinquire
StandardRange	15.6pg/ml-1000pg/ml(cellculturesupernates),7.8pg/ml-500pg/ml(mouseserum,plasma).Testhasasensitivityof<1="">
SampleType	ThiskitisusedforquantitativedetectionofmouseTNFalphaincellculturesupernates,serumandplasma(heparin,EDTA).
Specimen/Volumes	RefertoManual
SwissProtNumber	P06804
Concentration	LotSpecific
Applications	ELISA
GINumber	21926
Stability	Fourmonthsat4Candeightmonthsat-20C.
Reactivity	ReactivetoMouse.Thereisnodetectablecross-reactivitywithotherrelevantproteins.
KitSize	1x96wellplate
Storage	Storeat4°Cfor6months,at-20°Cfor12months.Avoidmultiplefreeze-thawcycles(Shippedwithwetice.)
IntendedUse	ResearchUseOnly