GenWay/Human NGF/NGF beta ELISA Kit/GWBZZD019/1x96 well plate

Description:

Short Description: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Additional Information:

Name	Human NGF/NGF beta ELISA Kit
Related Product Names	Beta nerve growth factor|Beta NGF|HSAN5|MGC161426|MGC161428|Nerve growth factor (beta polypeptide)|Nerve growth factor beta polypeptide|Nerve growth factor beta subunit|NGFB|NID67
Gene Symbol	NGF
Gene Fullname	nerve growth factor
Gene id	4803
Alias Symbols	Beta-nerve growth factor, NGF, NGFB
Protein Name	Beta-nerve growth factor
Description of Target	Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensor
Swissprot ID	P01138
Protein Accession Num	NP_002497.2
Nucleotide Accession Num	NM_002506.2
Species Reactivity	Human
Sensitivity	< 1="">
Kit Range	15.6 pg/mL-1,000 pg/mL
Immunogen	NSO, S122-A241
Standard Range	15.6pg/ml-1000pg/ml. Test has a sensitivity of < 1="">
Sample Type	cell culture supernates and serum
Specimen/Volumes	Refer to Manual
Swiss Prot Number	P01138
Specificity	There is no detectable cross-reactivity with other relevant proteins.
Concentration	Lot Specific
Applications	ELISA
GI Number	4803
Stability	Four months at 4C and eight months at -20C.
Reconstitution and Storage	Store at 4C for frequent use, at  -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Reactivity	Reactive to Human. There is no detectable cross-reactivity with other relevant proteins.
Kit Size	1x96 well plate
Kit Principle	Genway Biotech NGF ELISA Kit (Human) (GWB-ZZD019) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for NGF has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, S122-A241) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NGF is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human NGF captured in well.
Kit Component	Component Amount Lyophilized recombinant human NGF standard 10 ng/tube x 2 Anti-human NGF Antibody Well Plate 96 Wells Sample diluent buffer 30 mL Biotinylated anti- human NGF antibody 130 uL, dilution 1:100 Antibody diluent buffer 12 mL Avidin-Biotin-Peroxidase Complex (ABC) 130 uL, dilution 1:100 ABC diluent buffer 12 mL TMB color developing agent 10 mL TMB stop solution 10 mL
Kit Detection	Colorimetric, OD 450 nm
Kit Duration	~ 3 Hours
Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Lead Time	Domestic: within 1-2 weeks delivery International: 1-2 weeks
Intended Use	Research Use Only
Key Reference	1. Ullrich, A.; Gray, A.; Berman, C.; Dull, T. J. Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature 303: 821-825, 1983. 2. Sanico, A. M.; Stanisz, A. M.; Gleeson, T. D.; Bora, S.; Proud, D.; Bienenstock, J.; Koliatsos, V. E.; Togias, A. Nerve growth factor expression and release in allergic inflammatory disease of the upper airways. Am. J. Resp. Crit. Care Med. 161: 1631-1635, 2000.
Additional Information	Range: 15.6pg/ml-1000pg/ml
::	Principle: Aviva’s human NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards (NSO, S122-A241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NGF amount of sample captured in plate.
::	Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot experiment using standards and a small number of samples is recommended. 2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case. 3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes. 4. Duplicate well assay is recommended for both standard and sample testing. 5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate. 6. Don’t reuse tips and tubes to avoid cross contamination. 7. To avoid to use the reagents from different batches together. 8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before using. Background: Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2 The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.