GenWay/Caspase6 Colorimetric Assay Kit 200 assays/GWBAXR045/200 assays

Description:

Description:ActivationofICE-familyproteases/caspasesinitiatesapoptosisinmammaliancells.TheCaspase-6ColorimetricAssayKitprovidesasimpleandconvenientmeansforassayingtheactivityofcaspasesthatrecognizethesequenceVEID.Theassayisbasedonspectrophotometricdetectionofthechromophorep-nitroanilide(pNA)aftercleavagefromthelabeledsubstrateVEID-pNA.ThepNAlightemissioncanbequantifiedusingaspectrophotometeroramicrotiterplatereaderat400-or405nm.
Summary:
-Detectionmethod-Absorbance(400or405nm)
-Sampletype-Cellandtissuelysates
-Speciesreactivity-Mammalian
-Kitsize-Convenientsizes(25,100,200,400assays)
-Applications-Detectearly/middlestagesofapoptosis;differentiateapoptosisfromnecrosis.
Kitcomponents:
-CellLysisBuffer
-2XReactionBuffer
-VEID-pNA(4mM)
-DTT(1M)
-DilutionBuffer
FeaturesandBenefits:
-Simpleone-stepprocedure;takes1-2hours
-Fastandconvenient
-ComparisonoftheabsorbanceofpNAfromanapoptoticsamplewithanuninducedcontrolallowsdeterminationofthefoldincreaseincaspase-6activity
ShippingConditions:Gelpack

AdditionalInformation:

Name	Caspase-6ColorimetricAssayKit-200assays
RelatedProductNames	caspase6,caspase6,Caspase-6,Caspase-6ColorimetricAssayKit,Caspase-6,Caspase-6Substrate,VEID,VEID-pNA,Caspase-6assay,Caspase-6ColorimetricAssay
Format	AssayKit
Storage	-20C
IntendedUse	ResearchUseOnly