Lucigen/FastLink™ DNA Ligation Kit/LK0750H/50 Ligations

Benefits

• Cohesive-end ligations in 5 minutes at room temperature (Fig. 1).
• Blunt-end ligations in 15 minutes at room temperature (Fig. 2).
• Ligation of PCR product with A-overhangs in 1 hour at 16°C.
• High ligation efficiency (Table 1).
• Desalting of ligation products is not needed prior to transformation of electrocompetent or chemically competent cells.
• BAC ligation with inserts >100 kb can be performed in 4 hours, instead of overnight reactions as with other ligases.

Figure 1. Time course for cohesive end ligation using the Fast-Link™ Kit. Lambda Hind III markers were ligated using 2 units of Fast-Link DNA Ligase (Lanes 2-6). Lane M, Kilobase ladder; lane 1, no enzyme.

Figure 2. Time course for blunt-end ligation using the Fast-Link™ Kit. pUC 19 DNA digested with Pvu II was ligated using 2 units of Fast-Link DNA Ligase (lanes 1-6). Lanes M, Kilobase ladder.