Reliablyjoindouble-strandednucleicacidchains.
T4DNALigasecatalyzestheformationofaphosphodiesterbondbetweentheterminal5′phosphateand3′hydroxylgroupsofduplexDNAorRNA.TheenzymeefficientlyjoinsbluntandcohesiveendsandrepairssinglestrandednicksinduplexDNA,RNAorDNA/RNAhybrids.
T4DNALigaseVersion | Sizes | LigaseConcentration | ProvidedBuffer |
LowConcentrationLigase | 1,500U | 2U/µL | 10XT4DNALigaseBuffer |
HighConcentrationRapidKit | 1,500U 7,500U | 10U/µL | 2XRapidLigationBuffer, 10XT4DNALigaseBuffer |
10XT4DNALigaseBufferiscomposedof500mMTris-HCI,100mMMgCl2,50mMdithiothreitol,10mMATP,pH7.6@25°C.
2XRapidLigationBufferiscomposedof132mMTris-HCI,20mMMgCl2,2mMdithiothreitol,2mMATP,15%PEG,pH7.6@25°C.
Figure1: Purity |
Figure2: ExonucleaseandEndonucleaseActivityAssay |
UnitDefinition:OneWeissUnitisdefinedastheamountofenzymerequiredtoconvert1nmolof32P-labeledinorganicpyrophosphateintoNoritadsorbablematerialin20minutesat37°C,usingspecifiedreactionconditions.Note:1WeissUnitisapproximately67cohesiveendunits.
Source:ArecombinantE.colistraincarryingtheclonedT4DNALigasegene.
Storagebuffer:T4DNALigaseissuppliedin10mMTris-HCl,50mMKCl,1mMdithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%glycerol,pH7.5@25°C.
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