Excellentstranddisplacement,fidelityandspeed.
phi29DNAPolymeraseisahighlyprocessiveDNApolymerasewithapowerfulstranddisplacementactivityanda3′→5′proofreADIngexonucleasefunction.Theenzymeiscapableofupto70,000insertionsperbindingevent.
Anexampleofthesavingsyoucanexpect. |
Specifications
UnitDefinition:1unitisdefinedastheamountofpolymeraserequiredtoconvert0.5pmolofdNTP"sintoacidinsolublematerialin10minutesat30°C.
Source:ArecombinantE.colistraincarryingthephi29DNAPolymerasegenefrombacteriophagephi29.
UnitConcentration | 10,000U/mL |
Purity(SDS-PAGE) | >99% |
SSExonuclease | Functional |
E.coli16SrDNAContamination | 100U<10copies |
Endonuclease | 100U<10%converted |
Storage | -20&°C |
Suppliedat10,000U/mLin10mMTris-HCl,100mMKCl,0.1mMEDTA,1mMdithiothreitol,0.5%Tween-20,0.5%NP-40,50%glycerol,pH7.4@25°C.Alsosuppliedis10Xphi29DNAPolymeraseBuffercomposedof500mMTris-HCl,100mM(NH4)2SO4,40mMDithiothreitol,100mMMgCl2,pH7.5@25°C.10,000Uissuppliedas5×2,000U.
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