Lucigen/EconoTaq DNA Polymerase (separate Mg++)/300323/10,000 U (10 X 1,000 U)

GreatTaqPolymeraseperformanceatalowprice.
Choiceofreactionbuffers,withorwithoutMgCl₂.
Non-proofreadingPolymerase

FreeSampleAvailable

At$96for1000unitslistprice(quantitydiscountsavailable),EconoTaq’slowpriceiscoupledwithhighqualityandperformance.

QCspecificationsforEconoTaqarerigorous:

Greaterthan99%purebySDSgelelectrophoresis(seeFigure1).
NodetectableDNAcontaminationasdeterminedbyPCRusinggenericprimers.
Nodetectableendonuclease(nicking)activity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofsupercoiledpBR322DNAfor16hoursat70°Cresultsinnodetectableconversiontorelaxedorlinearformsbyagarosegelelectrophoresis.
Nodetectableexonucleaseactivity.Incubationof10UofEconoTaqDNAPolymerasewith1µgofHindIII-cutlambdaDNAfor16hoursat70°Cresultsinnosmearingofbandsonagarosegels.

EconoTaqPerformance
AsshowninFigures2and3Lucigen’sEconoTaqDNAPolymeraseperformsaswellas,orbetterthan,moreexpensiveTaqpreparationsfromseveralothersuppliersinroutinePCR.EconoTaqisaseffectiveinDNAamplificationasanotherstandardPCRenzyme,TflDNApolymerase(Figure4).Inthiscase,thebackgroundofnon-specificamplificationwasmuchlowerwithEconoTaq(compare“+”and“–“lanesforEconoTaqandTflinFigure4).EconoTaqDNAPolymerasealsooffershighlot-to-lotreproducibilityandreliability(Figures2and4)


Figure1.HighpurityofEconoTaqDNAPolymerase(SDSPAGE).Lane1,broadrangemolecularweightMarkers;Lane2,LucigenEconoTaqDNAPolymerase	Figure2.TaqDNApolymerasefromPromegaandNewEnglandBiolabswerecomparedtoLucigen’sEconoTaqDNAPolymerase(2differentlots)inamplifyingtheampicillingene(0.8kb)inapUC19vector.(–),noDNA.(+),DNAadded(40ng).MW,1kbladder.

EconoTaq Comparison AmpliTaq

Figure3.EconoTaqvs.AmpliTaq®(AppliedBiosystems)DNApolymeraseingenotyping.AllPCRreactionswereperformedinaRoboCycler96(Stratagene).Hip1genotypingwasperformedusingthefollowingPCRconditions:94°Cfor1min,35cyclesof94°Cfor30sec,62°Cfor60sec,72°Cfor90sec,and72°Cfor7min.Shh,CdoandGas1genotypingwereperformedusingthefollowingPCRconditions:94°Cfor2min,35cyclesof94°Cfor60sec,65°Cfor60sec,72°Cfor90sec,and72°Cfor7min.AllPCRreactionscontainedfinalconcentrationsof1µMofeachprimer,200µMdNTPs,1Xcresolredloadingdye,and1UoftheindicatedTaqpolymerase.SequencesforallPCRprimershavebeenpreviouslypublished(referencesavailable).
DatacourtesyofDr.BenjaminAllen,Dept.Molecular&CellularBiology,HarvardUniversity.

EconoTaq Tfl Comparison

Figure4.PCRamplificationwasperformedunderstandardconditionsusingthreedifferentlotsofEconoTaqDNAPolymeraseandbuffer,orduplicatereactionswithTflDNApolymeraseandbuffer(Promega).Reactionscontainedprimersspecificforthe16SribosomalRNAgene,withBacillusgenomicDNA(+)ornoDNA(-)asatemplate(1450bpproductexpected).

PleaseNote:
SomeapplicationsinwhichLucigen'sEconoTaqDNAPolymerasecanbeusedmaybecoveredbypatentsissuedandapplicableintheUnitedStatesandcertainothercountries.Becausepurchaseofthisproductdoesnotincludealicensetoperformanypatentedapplication,usersofthisproductmayberequiredtoobtainapatentlicensedependingupontheparticularapplicationinwhichtheproductisused.ThePCRprocessisthesubjectofEuropeanPatentNos.201,184and200,262ownedbyHoffman-LaRoche.ThosepatentsexpiredonMarch28,2006.ThecorrespondingPCRprocesspatentsintheUnitedStatesexpiredonMarch29,2005.Itisthesoleresponsibilityofthebuyertoensurethatuseoftheproductdoesnotinfringethepatentrightsofthirdparties.

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EconoTaqDNAPolymeraseisprovidedwithachoiceof10XReactionBufferwithMg++(“withMg++”);or10XReactionBufferwithoutMg++andaseparatetubeof25mMMgCl₂(“separateMg++”).PleaseinquireforBulkpricing!

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Lucigen/EconoTaq DNA Polymerase (separate Mg++)/300323/10,000 U (10 X 1,000 U)

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