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Lucigen/RNase I, E. coli/N6901K/1,000 Units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Lucigen/RNase I, E. coli/N6901K/1,000 Units

RemoveunwantedRNAbydigestingwiththisheatlABIle,non-sequencespecificribonuclease

  • Epicentre ProductComplete:UnlikeRNaseA,RNaseIdoesnothaveabasepreferenceanddigestsbetweenalldinucleotidepairsinsingle-strandedRNA
  • HeatLabile:RNaseIiseasilyinactivatedbyheating70°Cfor15minutes

RNaseIdegradessingle-strandedRNAtonucleoside3´monophosphatesvia2´,3´cyclicmonophosphateintermediatesbycleavingbetweenalldinucleotidepairs,2,3unlikeRNaseA,whichcleavesonlyaftercytosineanduridine.Inaddition,theenzymeiscompletelyinactivatedbyheatingat70°Cfor15minutes,eliminatingtherequirementtoremovetheenzymepriortomanysubsequentprocedures.

Applications

  • RemovalofRNAfromDNApreparations.1
  • RNaseprotectionassaystodetectsingle-basepairmismatchesinRNA:RNAandRNA:DNAhybrids.1

UnitDefinition:OneunitofRNaseIdegrades100ngofE.coliribosomalRNApersecondintoacid-solublenucleotidesat37°Cunderstandardassayconditions.

DilutionandStorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,and0.1mMEDTA.

QualityControl:RNaseIisfreeofdetectableexo-andendodeoxyribonucleaseactivities.

References

  1. Johnson,M.(1996)EpicentreForum3(4),7.
  2. Shen,V.andSchlessinger,D.(1982)TheEnzymesXV(PartB),501.
  3. delCardayré,S.B.andRaines,R.T.(1995)Anal.Biochem.225,176.

 

Figure1.RemovalofRNAfromplasmidDNApreparations.PlasmidDNAwaspreparedfrom1.5mLofanovernightculture,sUSPendedin50µLTEbuffer(10mMTris-HCl(pH8),1mMEDTA),andtreatedwithRNaseA,RNaseI,ornoenzyme.FiveµLofeachreactionwereresolvedbyagarosegelelectrophoresisandvisualizedbystainingwithethidiumbromide.Lane1,noRNase;Lane2,1.5UofRNaseI;Lane3,20µg/mLRNaseA;MW,1-kbladder.ArrowdenotessmallundigestedRNA.

ORDERINFORMATION

Providedwithdilutionbuffer,10XTNEbuffer,and0.1MDTT.
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