Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/600523/24 rxns (SixPacks)

Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)
Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.
Availableinarangeofhightransformationefficiencies(5×10⁹to4×10¹⁰cfu/µg).
Convenientpackagingoptions.

Lookingforchemicallycompetentversion?

FreeSampleAvailable

Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliability(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.

Choiceofstrain:

E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.

Choiceofefficiency:

E.cloni10GSUPREMEElectrocompetentCells>4×10¹⁰cfu/µgpUCDNA
SUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossible.
E.cloni10G&10GF´ELITEElectrocompetentCells>2×10¹⁰cfu/µgpUCDNA
ELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice.
E.cloni10GCLASSICElectrocompetentCells>5×10⁹cfu/µgpUCDNA
CLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.

CustomCompetentCells

BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.

Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleadingsupplier(Figures1and2)


Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16).		Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells)

Table1.E.cloniCompetentCellsforCloningandLibraryConstruction

**E.cloniCellLines**	TransformationEfficiency (cfu/µgpUCDNA)	CloningMethylatedDNA	BAC,CosmidCloning	Blue/WhiteScreening
10GSUPREMEElectrocompetent	>4×10¹⁰	YES	YES	YES withoutIPTGinduction
10GELITEElectrocompetent	>2×10¹⁰	YES	NO	YES withoutIPTGinduction
10GCLASSICElectrocompetent	>5×10⁹	YES	NO	YES withoutIPTGinduction
10GF´ELITEElectrocompetent	>2×10¹⁰	YES	NO	YES IPTGinductionrequired

Efficiencies

E.cloni 10GSUPREMEElectrocompetentCells > 4×10¹⁰ cfu/µgpUCDNA
E.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 10¹⁰ cfu/µgpUCDNA
E.cloni 10GCLASSICElectrocompetentCells > 5 × 10⁹ cfu/µgpUCDNA

ORDERINFORMATION

CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.

PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.

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Lucigen/E. cloni 10G ELITE Electrocompetent Cells > 2 × 10^10 cfu/µg/600523/24 rxns (SixPacks)

Choiceofstrain:

Choiceofefficiency:

ORDERINFORMATION