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Lucigen/DNATerminator® End Repair Kit/400352/50 rxns
  • Repairshearedorrestriction-digestedDNAends
  • Increasecloningefficiencyasmuchasa500%
  • Optimizedsystemforconsistent&reliableresults

RepairDNAends

Double-strandedDNAfragmentsforlibraryconstructionorlarge-scalesequencingareoftengeneratedbymechanicallyshearinglarger(genomic)DNA,aprocessthatprimarilyleavesunevenends.LargeDNAmayalsobecutwithrestrictionenzymestogeneratesmallerfragmentsforsubcloning.ShearedorrestrictedDNAfragmentsmustbe“end-repaired”beforeligationintoblunt-endcloningvectors(e.g.,Lucigen’sCloneSmart®,CopyRight®,orBigEasy®systems).

TheDNATerminatorEndRepairKitcreatesblunt,5'phosphorylatedendsonanytypeofshearedorrestriction-digestedDNAfragment,ensuringthehighestpossiblecloningefficiency(Figure1).

DNA Terminator Kit

Figure1.EffectofDNAshearingandend-repairmethodsonlibraryconstructionefficiency.Shotgunlibrarieswereconstructedusing250ngoflambdaDNAshearedbysonication(Son)orbytheGeneMachines®HydroShear™device(HS).ShearedDNAwasrepairedwithT4DNApolymeraseandKlenowfragment(TK)orDNATerminatorEndRepairKit(DT)andclonedusingLucigen’sCloneSmart®system.Valuesarecolonyformingunits(cfu)perlibrary.

ORDERINFORMATION

TheDNATerminatorEndRepairKitcontains:DNATerminatorEndRepairEnzymeMix,EndRepairBuffer,andacompleteprotocol.

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