Lucigen/NxGen® T7 RNA Polymerase/302232/125,000 U

T7RNAPolymerasecatalyzesthe5′→3′RNAsynthesisfromtheT7promoter.ItisaDNA-dependentRNApolymeraseclonedfromtheT7bacteriophage,whichrecognizestheT7promoterandterminatorsequenceswithhighspecificity.

Specifications

UnitDefinition:Oneunitisdefinedastheamountofenzymethatwillincorporate1nmolofATPintoacid-precipitablematerialin1hourat37°C.

Source:PurifiedfromastrainofE.colithatexpressestherecombinantT7RNAPolymerasegene.

UnitConcentration	50,000U/mL
Purity(SDS-PAGE)	>99%
SSExonuclease	500U<0.1%released
DSExonuclease	500U<0.1%released
Endonuclease	500U<10%converted
RNAseContamination	500U=nonedetected
E.coli16SrDNAContamination	500U<10copies
Storage	-20°C

ORDERINFORMATION

Suppliedat50,000U/mLin50mMTris-HCl,100mMNaCl,1mMdithiothreitol,1mMEDTA,50%glycerol,0.1%TritonX-100,pH7.9@25°C.Alsoprovidedis10XT7RNAPolymeraseBufferwhichin1Xformiscomposedof40mMTris-HCl,6mMMgCl₂,10mMDithiothreitol,2mMSpermidine,pH7.9@25°C.125,000Uissuppliedas5×25,000U.