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Lucigen/Sygnis SunScript™ One Step RTPCR Kit/SYG430025/25 rxn

Anovelhigh-temperatureOneStepRT-PCRkitbasedonthecombinationofSunScript™ReverseTranscriptaseRNaseH-andhigh-qualityTaqPolymeraseforthereversetranscriptionandPCRamplificationofspecificRNAfragmentsinoneconvenientsinglestep.

  • Improvedthermalstabilityforthereversetranscriptionstep: Worksupto85°C(60-65°Crecommended).
  • TranscriptionandamplificationofdifficultRNAs:TranscribesRNAswithhighGCcontentorcomplexsecondarystructures.
  • Greatsensitivity: StartswithpicogramamountsofRNA
  • ImprovedPCRspecificity:HotstartTaqpolymerasegreatlyimprovesspecificityduringamplification
  • HighyieldsofamplifiedDNA
  • Highreproducibilityacrossreactions: Bestchoiceforprocessingmultiplesampleswithminimumdeviations.
  • Easyhandling: Reagentsareaddedinasinglestep,whichalsominimizesdangersofcontaminationandreducesoverallhandlingtime.

TheSunScript™OneStepRT-PCRKitisaneasyandreliablesystemdesignedforfast,specificandsensitiveendpointRT-PCRreactions.ThiskitcontainsallthecomponentsneededtoperformbothreversetranscriptionandPCRamplificationinasingletubebyusinggenespecificprimersina“onestep”reaction.Thisminimizescontaminationsandconstitutesaconvenientsystemtoprocessmultiplesamples.

  • OneStepRT-PCRProtocolOverview
  • DetectionofmRNAswithdifferentexpressionlevels
  • HighthermalactivityoftheRTstep
  • Highsensitivity
  • Highreproducibility
  • AmplificationanddetectionofdifficultRNAtargets

OneStepRT-PCRProtocolOverview

Overview of One Step RT PCR

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DetectionofmRNAswithdifferentexpressionlevelsDetection of mRNAs with different expression levels

100ngoftotalRNAfromHeLacellswerereversetranscribedandamplifiedwithSunScript™OneStepRT-PCRKitandspecificprimersfortheindicatedgenes(GAPDH,HERCGUSB,CLTCandACTIN).

Thekiteasilydetectsgeneswithvastlydifferentexpressionlevels.

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HighthermalactivityoftheRTstep

High thermal activity of the RT one step

100ngoftotalRNAfromHeLacellswerereversetranscribedandamplifiedwithSunScript™OneStepRT-PCRKitandspecificprimersfortheGAPDHopenreadingframe(productlength305bp).

TheRTreactioncanbeperformedattemperaturesupto85°C,makingthisthebestchoicefordetectingdifficultRNAs,withhighdegreesofsecondarystructure.

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Highsensitivity

High Sensitivity One Step PCR Data

TheindicatedamountsoftotalRNAfromHeLacellswerereversetranscribedandamplifiedwithSunScript™OneStepRT-PCRKitandspecificprimersforGAPDHcDNA(productlength305bp).

ThesensitivityofthekitisgoodenoughtodetectexpressedgenesfromtotalRNAstartingmaterialintheorderofpicograms.

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Highreproducibility

Highly Reproducible Data

100ngoftotalRNAfromHeLacellswerereversetranscribedandamplifiedwithSunScript™OneStepRT-PCRKitandspecificprimersfortheindicatedgenes(4replicatesareperformedpergene).

Thefourindependentreplicatespergeneshowsimilarsensitivityandyield,makingthiskitthebestchoiceforprocessingmultiplesampleswithminimumdeviations.

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AmplificationanddetectionofdifficultRNAtargets

Amplification and detection of difficult RNA targets data

100ngoftotalRNAfromHeLacellswerereversetranscribedandamplifiedwithSunScript™RT-PCRKitandfourdifferentspecificprimerpairsforafragmentoftheTAFII100cDNAwith75%GCcontent(productlengths430-470bp).

TheRT-PCRprotocolwithsampledenaturationwasperformedhereandthecDNAsynthesisstepwasperformedat60°C.
ThefouroligopairsproducedeffectiveamplificationbandsdespitetheelevatedGCcontentofthetemplateRNA.

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ORDERINFORMATION

EachSunScript™OneStepRT-PCRKitcontains:EnzymeMix,2XReactionBufferandwater.ThecompletemanualisavailableundertheManualtab.LucigenisanauthorizeddistributorofSygnisproductsintheUS.

SunScript™OneStepRT-PCRKitisintendedformolecularbiologyuseonlyandinvitrouseonly.Thisproductisnotintendedfordiagnosis,preventionortreatmentofadiseaseinhumanbeingsoranimals.


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