Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BACOptimized Replicator v2.0 Electroco

Efficientlycloneanysizeinsertupto200kb.
CreatelibrariesfromA/T-orG/C-richgenomes.
Clonegeneclustersoroperons.
Highinsertstability.
Induciblecopynumber.

Lucigen'sCopyRight®v2.0BACcloningkitswithpSMART®BAC(Figure1)orpEZBAC(Figure2)vectorandBAC-OptimizedReplicator™v2.0ElectrocompetentCellsresultinunprecedentedaccuracy,reliability,andefficiencyinBACcloningandlibraryconstruction.ThesevectorsincorporatetheCloneSmart®transcription-freetechnology,whichminimizescloningbiasandenablescloningofotherwisetoxicgenes,alongwithinduciblecopynumberamplification(20-50X)forhighDNAyieldsandalacZ-sacBstufferfragmentthateliminatesuncutvectorfromrecombinants,resultinginzerobackground. UnlikeotherBACcloningsystems,Lucigen'sCloneSmarttechnologyassuresthateven"unclonable"insertsarestablymaintained.Sequencesarenotlostorrearrangedinthecloningprocess. CopyRightKitsoffersuperiorperformanceforBACcloningatamuchbetterpricethanotherkits. Figure3showsanexampleofaRandomShearBAClibrarygeneratedwithpSMARTBAC.


Figure1.CopyRightvectorpSMARTBAC. ori2,repE,IncC-single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C-partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lambdapackagingsignal;T-CloneSmarttranscriptionterminators;sacB-sucrasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.	Figure2.CopyRightvectorpEZBAC.ori2,repE,IncC,single-copyoriginofreplication;oriV-inducibleoriginofreplication;parA,B,C,partitiongenes;Cm^r-chloramphenicol-resistancegene;cosN-lambdapackagingsignal;lacZ-alphapeptideofthebetagalactosidasegene.Approximatepositionsofsequencingprimersandtranscriptionterminatorsareindicated.

Arabidopsis BAC gel

Figure3.ArabidopsisRandomShearBACLibraryinthepSMARTBACvector.7,680cloneswereobtained,withanaverageinsertsizeof105kband~5Xgenomecoverage.

ConvenientSuccess
Efficientlyobtainyourcloneorcreateyourlibrary—theCopyRightv2.0CloningKitseliminatetediousvectorandcompetentcellpreparation,aswellastime-consumingQCexperiments. Kitsincludeoptimizedreagents,ligation-readyvector,highlyefficientelectrocompetentcells,detailedinstructions,andatrouble-shootingguide.

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EachCopyRight®v2.0BACCloningKitcontains:pre-cutvector(pSMARTBACwithachoiceofBamHIorHindIIIsites),CopyRightDNALigase,CopyRight5XLigationBuffer,PositiveControlInsertDNA,SequencingPrimers,BAC-OptimizedReplicatorv2.0ElectrocompetentCellsinDUOpackaging(2transformationspertube),PositiveControlInsertDNA,RecoveryMedium,ArabinoseInductionSolution,andcompleteprotocols.EachCopyRightpEZBACCloningKitcontains:pre-cutCopyRightvector(pEZBACBlunt),CloneSmartDNALigase,CloneDirect™10XLigationBuffer(includesATP),PositiveControlInsertDNA,ReplicatorInductionSolution,SequencingPrimers,andcompleteprotocols.KitswithcellsalsoincludeBAC-OptimizedReplicatorv2.0ElectrocompetentCells(>1×10¹⁰ cfu/µgDNA)packagedintwotransformationspertube(DUOs),PositiveControlPlasmid,andRecoveryMedium.

Pleasenotethatthefollowingvectorshavebeendiscontinued:pSMARTBACEcoRI(catalognumbers42031-1,42031-2)andpEZBACBamHI(catalognumbers42007-1,42007-2and42012-1).”

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Lucigen/CopyRight v2.0 BAC Cloning Kit, pSMART BAC HindIII w/BACOptimized Replicator v2.0 Electroco

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