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Lucigen/T4 DNA Polymerase, Exonuclease Minus/300853/1500 U188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Lucigen/T4 DNA Polymerase, Exonuclease Minus/300853/1500 U

  • OnlysourceforrecombinantT4DNAPolymerase,ExonucleaseMinus.
  • PotentialapplicationsincludeenhancingDNAsequencingreads.

T4DNAPolymerase,ExonucleaseMinusisaDNA-dependentDNApolymerasewhichcatalyzesthe5’→3’synthesisofDNAandrequirestemplateandprimer. Additionally,theenzymelacks3’→5’exonucleaseactivity.

Source:
PurifiedfromastrainofE.colithatcarriesaT4DNAPolymerase,ExonucleaseMinusexpressionplasmid.

Applications:

  • DNASequencingreaction.(1)

Heatinactivation:70°Cfor15minutes.

Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.

AdditionalInformation:

  • MayincreasetheDNAsequencingread-lengths.

References

  1. NancyG.Nossal(1998)Genetics148:1535-1538.

ORDERINFORMATION

RecombinantT4DNAPolymerase,ExonucleaseMinusissuppliedataconcentrationof3,000U/mlinastoragebufferof50%glycerol,100mMKPO4(pH6.5)and1mMDTT.Alsosuppliedis10XDNAPolymeraseBufferAcomposedof100mMTris-HCl(pH7.9),100mMMgCl2,500mMNaCland10mMDTT.

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