Lucigen/MasterAmp™ Tth DNA Polymerase/TTH72250/250 Units

MasterAmp™TthDNAPolymeraseisarecombinantDNAenzymefromThermusthermophilusthathasbothDNA-dependentandRNA-dependent(i.e.,reversetranscriptase)DNApolymeraseactivitiesupto~95°C.MasterAmpTthDNAPolymeraseishighlypurifiedusingaproprietaryprocedurethatvirtuallyeliminatescontaminatingbacterialDNA.

Applications

• PCRamplificationofDNAandone-stepRT-PCRofRNA.
• PCRamplificationofRNAandDNAtemplateshavingahighdegreeofsecondarystructure.

Optimizationof[Mg²⁺]and[Mn²⁺]:MagnesiumandmanganeseionconcentrationsshouldbeoptimizedforDNAsynthesis.TheoptimalconcentrationofeachmetalcationishighlydependentonthedNTPconcentrationandonthetemplate,primers,andprotocolused.Formanytemplates,theoptimalconcentrationofmagnesiumionsusingMasterAmpTthDNAPolymeraseisapproximately1.5mMiftheconcentrationofeachdNTPinthereactionis0.2mM.Ifusedwith1.5mMMg²⁺,theoptimalconcentrationofMn²⁺forRNA-dependentDNAsynthesisisapproximately0.5mMformanytemplates.ForanRT-PCRreaction,werecommend3.0mMMg²⁺and0.5mMMn²⁺formosttemplates.Alternatively,werecommendtheMasterAmp™PCROptimizationKitwithammoniumsulfateforrapidPCRoptimization.

UnitDefinition:OneunitofMasterAmpTthDNAPolymeraseconverts10nmolofdNTPsintoacid-insolublematerialin30minutesat70°Cunderstandardassayconditions.

StorageBuffer:50%glycerolcontaining50mMTris-HCl(pH7.5),100mMNaCl,0.1mMEDTA,0.5%Tween20,0.5%NP-40,and1mMDTT.

QualityControl:MasterAmpTthDNAPolymeraseistestedfortheabsenceofdetectablenonspecificexo-andendonucleaseandRNaseactivities,andarefunctionallytestedinPCR.

*PurchaseofMasterAmp™TthDNAPolymeraseincludesanimmunityfromsuitunderpatentsspecifiedintheproductinserttouseonlytheamountpurchasedforthepurchasersowninternalresearch.Nootherpatentrights(suchas5′NucleaseProcesspatentrights)areconveyedexpressly,byimplication,orbyestoppel.FurtherinformationonpurchasinglicensesmaybeobtainedbycontactingtheDirectorofLicensing,AppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404,USA.Coveredbyissuedandpendingpatents.Forcompletelicensestatement,seep.iv.

**Coveredbyissuedand/orpendingpatents.