Lucigen/T4 Polynucleotide Kinase/300611/1,000 U

T4PolynucleotideKinasecatalyzesthetransferandexchangeofgamma-phosphateofATPtothe5´-hydroxylterminusofpolynucleotides(double-andsingle-strandedDNAandRNA)andnucleoside3´-monophosphates.

Source:
PurifiedfromastrainofE.colithatcarriesaT4PolynucleotideKinaseexpressionplasmid.

Applications:

• End-labelingDNAforprobesandDNAsequencing(1)
• Additionof5´-phosphatestooligonucleotidestoallowsubsequentligation(2)

Heatinactivation:70°Cfor15minutes.

Storage:
Enzymeandbuffershouldbestoredat-20°C.Enzymeisstablefor12monthsifhandledproperly.

AdditionalInformation:

• AmmoniumionsarepotentinhibitorsofT4PolynucleotideKinase. DNAshouldnotbeprecipitatedfromordissolvedinbufferscontainingammoniumsalts.
• Protruding5’-overhangsaremoreefficientlyphosphorylatedthanblunt-orrecessed5’overhangs.

References:

1. Sambrook,J.andRussell,W.(2001)MolecularCloning:ALaboratoryManual,(3rded.),9.55-9.56,9.68-9.69.
2. Sambrook,J.andRussell,W.(2001)MolecularCloning:ALaboratoryManual,(3rded.),13.16-13.17.