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Lucigen/AmpliScribe™ T7Flash™ Transcription Kit/ASF3507/50 Reactions

GeneratemaximumamountsofRNA-over180ugRNAfrom1ugDNAtemplate

  • Formerly from EpicentreSavetimewith30minutehigh-yieldprotocol
  • ScaleupthereactiontoproducegramsofRNA
  • SeeFAQ

TheAmpliScribe™T7-Flash™TranscriptionKitusesEpicentre'srapid,high-yieldinvitrotranscriptiontechnology.AnAmpliScribeT7-FlashTranscriptionreactionyieldsmoreRNA(180µgper20-µlreaction)in30minutesthanotherhigh-yieldinvitrotranscriptionkitreactionsproducein2hours(Fig.1),includingEpicentre'sownAmpliScribeT7HighYieldKit.TheAmpliScribeT7-FlashKitutilizesproprietaryenzymeformulationsthatenablethemaximumpossibleyieldsofRNAfromvirtuallyanytemplatethatisdownstreamoftheT7RNApolymerasepromoter,includingsequencesinlinearizedplasmids,double-strandedoligosorPCRproducts.

Features

  • Exceptionallyhighyields.Upto180µgoffull-lengthRNAcanbeproducedfrom1µgof1-kblinearizedplasmidDNAtemplate.
  • Scaleable.Reactionscanbescaleduptoobtaingrams(ormore)ofRNA.
  • Fast.AstandardAmpliScribeT7-Flashreactioniscompletein30minutes.
  • Convenient.Rnaseinhibitorincluded.
  • Trusted.Canbeusedforclassic"Eberwine"-typeRNAamplificationprocedures.

    • ORDERINFORMATION

    AmpliScribe™T7-Flash™EnzymeSolution,AmpliScribe™T7-Flash™10XReactionBuffer,ATP,CTP,GTPandUTPSolutions,RNase-FreeWater,RNase-FreeDNaseI,DTT,ControlDNATemplate(linearized),RiboGuard™RNaseInhibitor.

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