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Oxford Expression /pOET2C 6xHis Transfer Plasmid/2001032/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Oxford Expression /pOET2C 6xHis Transfer Plasmid/2001032/1 Ea

Description:

pOET2C6xHis(cat#2001032)(10ug)isabaculovirustransfervectordesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedrin(polh)promoter. ThevectorencodesanoptionalC-terminal6xHis-Tag®fusionsequencethatmaybeutilized. Thisgreatlyeasesthepurificationoftherecombinantproteinsincethe6xHis-containingfusionproteinsbindwithhighaffinitytoNi-NTAAgarose. pOET2C6xHishasaColE1originofreplicationandanampicillinresistancegeneforselectioninE.coli.Thepolhsequenceshavebeenreplacedbyamultiplecloningsite(MCS)containinguniquerestrictionenzymesitesforinsertionoftheforeigngeneinthecorrectorientation. TheAcMNPVsequencesflankingthegeneinthetransfervector’sMCSallowrecombinationwiththeviralDNAtoinserttheexpressioncassetteintothepolhlocus.pOET2C6xHisiscompatIBLewithanybaculovirussystemthatutilizeshomologousrecombinationininsectcells.

Variations

pOET1C6xHis hasthesameMCSaspOET2C6xHisexceptinthereverseorientation.

pOET2N6xHis hasanN-terminalratherthanC-terminal6xHistag.

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