Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
Rubicon Genomics/48 reactions/R300381,/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线
产品资料

Rubicon Genomics/48 reactions/R300381,/1 Ea

HighlyReproducIBLeCNVDetectionOvertheEntireGenome

Amplifiedlibrariesfrom11individualflow-sortedH929cellsweresequencedonanIlluminaMiSeq®anddownsampledto250,000totalreads.Thirty-fivebasesingle-endreadsweremappedtohumanovertheentiregenome.

HighlyReproducibleCNVDetectioninChromosome1

IntheeachoftheindividualH929cells(seepreviousslider),a22Mblossand30Mbgainwereconsistentlydetectedinchromosome1asindicatedbythepinkorbluebars.

PicoPLEXDNA-seqKitProvidesAneuploidyCallsConcordantwithArrays

PicoPLEXDNA-seqKitwastestedforequivalencyto24sure™arrays(Illumina)byusingthesamesingleembryocelllibraryforarrayandNGStesting.Librarieswerepooledingroupswith48compatiblebarcodes,andsequencedina2x100MiSeqv2PEexperiment.Sequencingresultswereconcordantwitharrayresultsandsequencingshowedaterminalgainin2p(showninfigure)andaterminallossin12q(datanotshown),whichwereconsistentwithanundetectedtranslocationinthepaternalDNAconfirmedbyhighresolutionFISHanalysis.(DataprovidedbyGIVF).

IndividualLibraryQuantificationEliminated

Flow-sortedunsynchronizedH929cellswereamplified,pooledwithconstantvolume,andloadedontoaMiSeqv3flowcell.QuantificationofthelibrarieswasunnecessarybeforepoolingduetothehighlyreproducibleamountofproductproducedbyPicoPLEXDNA-seqreactions.Thecolumnswhichprovidednoreadswerewellsinwhichthecellwasabsent.

PicoPLEXDNA-seqKitLibraryPreparationWorkflowforIlluminaNGSPlatforms

PicoPLEXDNA-seqKitSingle-TubeWorkflow

PicoPLEXDNA-seqKitTechnology

PicoPLEXDNA-seqKitusesthesametechnologyastheWGAkit:cellsarelysed,quasi-randomprimerspre-amplifytheDNAselectively,andafinalPCRamplificationaddstheIlluminabarcodes.

Storeat-20°C.

Guaranteedfor12monthsat-20°Cinaconstanttemperaturefreezer.

CAT.NO.R300381,48reactions

Thekitcontainseverythingneededtodo48reactions,including48dualindexesinasingleuse96-wellplate.

  • Reduceambiguity:highlyreproducibleCNVandaneuploidydetection
  • Reduceworkflow:fromasinglecelltoasequencing-readylibraryinthreesteps
  • Reducecost:asinglekitcontainseverythingneededtoprepareasequencing-readylibrary
  • Reducecontaminationanderror:libraryprepinasingletubeorwell,notransfersnecessary
  • Reducetimetoresults:IlluminaNGSlibrariespreparedinlessthan3hours
相关产品

matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY

intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15

goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP

novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg

novateinbio/SARS-Associated Coronavirus Spike Mosaic S ( C ) Recombinant ( SARS Mosaic S ( C ) )/500ug/PT_42025_500ug

matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB

intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24

goprolytix//10 ug, 100 ug/RTF-0300

最新签约

Liposoma

KAMIYA

Bioclone

intactgenomics

Abbexa

Emfret

Tribioscience

goprolytix

技术文章

ProChimia/HS(CH2)mCOOH/FT 002m101/1 g

Alomone经销商/代理商 Alomone蛋白质

prospec mSCF

【供应 GelRed核酸染料 （Biotium 41003）】价格_厂家 苏州蚂蚁淘

PDMS Device Fabrication and Surface Modification | Protocol

prospec HOR 250

上海正品NSJ Bioreagents生物试剂1 NSJ Bioreagents细胞生物学

杭州Enzymatics inc批发，产品货源 Enzymatics T4连接酶

新闻动态

苏州Enzymatics型号参数规格 Enzymatics超纯poly酶

19413561 GAMMASECRETASE INHIBITOR X 1MG

合肥中国Abbexa官网，Abbexa Ltd价格更新 abbexa授权代理

Abbexa中国采购 abbexa纯化蛋白

RhodamineDHPE美国/Biotium询价/索取资料——苏州蚂蚁淘

武汉美国Polysciences公司 分析试剂Polysciences

广州Anatrace型号参数规格 Anatrace膜蛋白提取与纯化

Genovis 特约代理苏州蚂蚁淘实验试剂有限公司

行业前沿

正品Pfenex生物试剂 重组白喉杆菌CRM197,为什么这么便宜?

PfenexX中国代理价格，厂家 SEA133鲑鱼纤维蛋白原,求推荐!

Pfenex中国采购 三文鱼凝血酶/鲑鱼凝血酶 Salmon Thrombin,是正品吗?

Polysciences聚乙烯亚胺 Polyethylenimine,好用吗?

PolysciencesX中国代理价格，厂家 Polysciences24765,哪里有卖?

Polysciences代理，产品目录 Polysciences聚合物,是正品吗?

Sea Run Holding产品促销 重组白喉杆菌CRM197,为什么这么便宜?

生命科学Pfenex价格优惠 Sea Run Holding代理，产品目录,有优惠活动吗?

最热产品

Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003

Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003

Qubit™ dsDNA BR Assay Kit Q32853现货

Parafilm M封口膜/PM996/4x125/整箱

Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销

LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货

GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g

Leica徕卡819一次性窄刀片【50片/盒，10盒/条】/14035838925

推荐新闻

RA Biosources报价单 rabios代理

Lampire型号参数规格 Lampire公司

品牌试剂实验Medica Lisotopes代理 Medica Lisotopes产品

进口试剂QoolAbs直采 Qoolabs噬菌体筛选

进口Molecular Research Center品牌清关代理 MRC一步法TRIzol RNA抽提

授权代理商，正品 Spherefluidics授权

Ursabioscience美元价/进货价 Ursa BioScience产品

Wards Science抗体，试剂，血清，细胞 wardsci授权

新闻动态
行业前沿
技术文章
最新产品