产品资料

Rubicon Genomics/ThruPLEX Tag-seq 96D Kit (96 reactions, 96 dual indexes)/R400586/1 Ea

ReduceBackgroundforResultsYouCanTrust

ThruPLEXTag-seqlibrarieswerepreparedfrom30ngofthe1%HorizoncfDNAstandard.FollowingAgilentSureSelecttargetenrichmentandsequencingtoachieve~10,000Xcoverage,datawasanalyzedwiththeCurioGenomicsbioinformaticsplatform.Usingtherawreads,theEGFRmutation(yellowdot)isobscuredbybackgrounderrors(left).ThemutationisreADIlyidentifiedwhenUMTsareutilizedindataprocessingtocorrectforerrors(right).

DramaticallyIncreasetheSignal-to-NoiseRatiotoDiscoverMore

Datafromeitherthe30ngHorizoncfDNAlibrary(previousslider)(A)oraThruPLEXTag-seqlibrarypreparedfrom10ngHorizoncfDNAstandard,enrichedwithacustom240KBpanel(NimbleGenSeqCapEZ)andsequencedonaHiSeq2500toachieve~5,000Xcoverage.AnalysiswasperformedusingtheCurioGenomicsbioinformaticsplatform.

Single-Tube,ThreeStepWorkflowHelpsyourPreciousSamplesGoFurther

Startingwith1to50ngofDNA,ThruPLEXTag-seqKitcreatesindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Nopurificationorsampletransferstepsarerequired.Thestreamlinedworkflowisperformedin2hoursinasingletubeorwell,preventingsamplelossandenhancingpositivesampleidentification.

ThruPLEXTechnologyGeneratesHighQualitylibrariesthefirsttime,everytime

ThruPLEXtechnologyisa3-stepreactionthatstartswithfragmenteddouble-strandedDNAorcell-freeDNAwhichisrepairedinahighlyefficientprocess.Backgroundisreducedusingdouble-strandedadaptorswithnosingle-strandedtails.Bluntendligationoccurswithhigh-efficiency.Blocked5"endsreduceadapter-adapterligation.

TimeSavingBioinformaticsSolutions

ReachresultsquicklywiththeCurioGenomicscloud-basedsoftware(www.curiogenomics.com)orConnor,anopen-sourcetoolavailablefordataprocessing(https://github.com/umich-brcf-bioinf/Connor).

Guaranteedfor9monthsat-20°Cinaconstanttemperaturefreezer.

Storeat–20°C.

CAT.NO.R400584ThruPLEXTag-seq6S Kit(12reactions,6singleindexes)

CAT.NO.R400585ThruPLEXTag-seq48SKit(48reactions,48singleindexes)

CAT.NO.R400586ThruPLEXTag-seq96DKit(96reactions,96dualindexes)

ThruPLEXTag-seqcontainsallnecessaryreagentsforpreparingindexedIlluminaNGSlibraries,includingoptimizedIllumina-compatIBLeadaptersandindexingreagents.

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