企业QQ
官方微信
ThruPLEXPlasma-seqKitgeneratedqualitylibrarieswithhighdiversityandalownumberofduplicatesandunmappedreads.Cell-freeDNAwasextractedfrom3plasmasamples,andlibrarieswerepreparedattheamountsindicatedasmeasuredbyqubit®.TheamountofmononucleosomalDNAineachsampleasmeasuredbytheBioanalyzer®correspondedto0.09ng,0.62ng,and15.44ng.PooledlibrariesweresequencedonanIlluminaNextSeq®500asapaired-endrunwith17Mto25Mreadsperlibrary,Duplicationrateswerecalculatedafterdown-samplingthedatato17Mreadsperlibrary.
ThruPLEXPlasma-seqKitlibrarieswerecapturedathighefficiencyandgenerateddatawithdeepcoverageofthekinomeformutationdetection.Librarieswerepreparedfrom3plasmasamplesatinputamountsof5ng,6.5ng,and10ngintriplicate,andtargetedsequencingwascarriedoutonanIlluminaMiSequsingsamplesenrichedwiththeClearSeqHumanDNAKinomePanelforSureSelectXT2.Onaverage,5Mreadsweregeneratedperlibrary.Selectedbasesweresuccessfullycapturedbasesthatwereinorwithin250bpofthebaits.
ThruPLEXPlasma-seqKitprovidedthemostreproducibleandunbiasedGCcoverageacrossthehumangenome.ThruPLEXlibrariesshowedminimalvariABIlityacross9individualplasmasamplestested.Librarieswerepreparedfromcell-freeDNAisolatedfrom1mLofplasmasamplesandsequencedonanIlluminaNextSeq500.FourseparateplasmasampleswereusedtoconstructtheNEBNextUltralibraries.
IlluminaNGSlibrarieswerepreparedwith5ngofcell-freeDNAisolatedfrompooledplasmasamplesusingThruPLEXPlasma-seqKit.Low-passWGSwasconductedonaHiSeqwithapproximately12.5Mreadsperlibrary.(DatacourtesyoftheKarolinskaInstitute,Sweden).
Startingwith1to30ngofcell-freeDNA,ThruPLEXPlasma-seqKitcreatesindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Nopurificationorsampletransferstepsarerequired.Thestreamlinedworkflowisperformedin2hoursinasingletubeorwell,preventingsamplelossandenhancingpositivesampleidentification.
ThruPLEXPlasma-seqKittechnologyisa3-stepreactionthatisoptimizedforcell-freeDNA.Cell-freeDNA(1ngto30ng)isfirstrepairedinahighlyefficientprocess.Backgroundisreducedusingdouble-strandedadapterswithnosingle-strandedtails.Blunt-endligationoccurswithhigh-efficiency.Blocked5’endsreduceadapter-adapterligation.Backgroundisfurtherreducedbydestroyingunusedadaptersafterligation.Ahigh-fidelityamplificationcompletesthereactiontogenerateindexedIlluminalibraries.
Guaranteedfor9monthsat-20°Cinaconstanttemperaturefreezer.
Storeat–20°C.
CAT.NO.R400490ThruPLEXPlasma-seq12S Kit(12reactions,12singleindexes)
CAT.NO.R400491ThruPLEXPlasma-seq48SKit(48reactions,48singleindexes)
CAT.NO.R400492ThruPLEXPlasma-seq96DKit(96reactions,96dualindexes)
ThruPLEXPlasma-seqcontainsallnecessaryreagentsforpreparingindexedIlluminaNGSlibraries,includingoptimizedIllumina-compatibleadaptersandindexingreagents.
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g