ThruPLEXDNA-seqKityieldedmorediverselibraries(left)withlessinputDNAandgeneratedfewerduplicates(right)at25Mreadpairsthanotherkits.NGSlibrarieswerepreparedwiththeindicatedamountofreferencegDNAusingthelibrarypreparationkitsindicatedandsequencedonaMiSeq,v3flowcell.
Startingwithaslittleas50pgoffragmenteddouble-strandedDNAorCDNA,ThruPLEXDNA-seqKitcreatesindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Thestreamlinedworkflowpreventssamplelossandisperformedinabout2hoursinasingletubeorwellpreventingsamplelossandenhancingpositivesampleidentification.
Relativecoverageofthehumangenomeasafunctionof%GC(50nginputDNA).(Left)ThruPLEXDNA-seqKithasexcellentcoveragethroughouttheentirerange.(Right)ThruPLEXDNA-seqKit(gold)performsaswellasorbetterthanotherkitstested.Notethattheoptimalcoverageshouldbeequalto1inthesegraphs.GCcompositiondistributionofthehumangenomeisrepresentedbytheredbars.
ThruPLEXDNA-seqKithasalowerandbroaderrecommendedinputrangethanotherkits.ThesampleinputamountforThruPLEXDNA-seqKitis20timesmoresensitivethanotherkits,rangingfrom50pgand50ng andrequiresnooptimizationoftheprotocoloradapterconcentration. Indexesandadaptersareincludedwiththekit.
ThruPLEXDNA-seqKitconvertsdouble-strandedDNAsamplestoindexedlibrariesin3simplesteps:endrepair,adapterligation,andhigh-fidelitylibraryamplification.Thestreamlinedworkflowpreventssamplelossandhandlingerrorsandisperformedinasingletubeorwellinlessthan2hours.
ThruPLEXtechnologyisa3-stepreactionthatstartswithfragmenteddouble-strandedDNA(0.05ngto50ng)whichisrepairedinahighlyefficientprocess.Backgroundisreducedusingdouble-strandedadaptorswithnosingle-strandedtails.Bluntendligationoccurswithhigh-efficiency.Blocked5’endsreduceadaptor-adaptorligation.Backgroundisfurtherreducedbydestroyingunusedadaptorsafterligation
Storeat-20°C.
Guaranteedfor9 monthsat-20°Cinaconstanttemperaturefreezer.
CAT.NO.R400406ThruPLEXDNA-seq48DKit(48reactions,48dualindexes)
CAT.NO.R400407ThruPLEXDNA-seq96DKit(96reactions,96dualindexes)
CAT.NO.R400427ThruPLEXDNA-seq48SKit(48reactions,48singleindexes)
CAT.NO.R400428ThruPLEXDNA-seq12S(48)Kit(48reactions,12singleindexes)
CAT.NO.R400523ThruPLEXDNA-seq6S(12)Kit(12reactions,6singleindexes)
ThruPLEXDNA-seqcontainseverythingneeded,includingindexesinabarcoded96-wellplate.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn