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ubpbio/GSTNEMO (UBAN)/I1531/500 µg
GSTwasfusedontheN-terminusofthelinearpolyubiquitinchainbindingdomainofNEMOen-compassingaminoacids183-339.ThisfusionproteincanbeusedforinvitroGSTpulldownas-saysandforenrichmentofcellularproteinsconjugatedwithlinearpolyubiquitinchainsinwholecellortissuelysates.GST-NEMO(UBAN)canbeprecipitatedusingglutathioneresin.Afterwashing,GST-NEMO(UBAN)anditsboundproteinscanbeelutedbyabuffercontaining10mMglutathione.

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GSTwasfusedontheN-terminusofthelinearpolyubiquitinchainbindingdomainofNEMOen-compassingaminoacids183-339.ThisfusionproteincanbeusedforinvitroGSTpulldownas-saysandforenrichmentofcellularproteinsconjugatedwithlinearpolyubiquitinchainsinwholecellortissuelysates.GST-NEMO(UBAN)canbeprecipitatedusingglutathioneresin.Afterwashing,GST-NEMO(UBAN)anditsboundproteinscanbeelutedbyabuffercontaining10mMglutathione.
Images:(Clickimagetoenlarge)
ProductName:GST-NEMO(UBAN)
AlsoKnownAs:IKBKG;IP;IP1;IP2;FIP3;IPD2;NEMO;FIP-3;Fip3p;AMCBX1;ZC2HC9;IKK-gamma
CatalogNo.:I1531
Size:500µg
MolecularWeight:18.4kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~90%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheet，MSDS
NCBIRefSeq:NM_001099857
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkers
Lane2:5µgpurifiedGST-NEMO(UBAN)
ShippingMethod:Dryiceshipping
References:1.vanWijkSJ,etal.(2013)NatProtoc.8(7):1449-58
2.vanWijkSJ,etal.(2012)MolCell.14;47(5):797-809
Coomassie-stainedSDS-PAGE
Lane1:Molecularweightmarkers
Lane2:5µgpurifiedGST-NEMO(UBAN)


Note:
1.NEMO(183-339)mayalsobindlongpolyubiquitinchainslinkedvialysine63.
2.Dissolve10mMglutathioneinaneutralbuffercoulddroppHto3-4.Use0.2MNaOHtoad-justpHbacktoneutral.

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