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ubpbio/Anti-Ubiquitin (P4D1)/Y3012/200 µg

Anti-UbMouseMonoclonalAntibody

AdditionalInformation

Details

Specificity
Recognizesmonoandpolyubiquitinatedproteins,freepolyubiquitinchainsandubiquitininawiderangeofspecies.Thisantibodyrecognizesdifferenttypesofpolyubiqutinchains.
IsotypeMouseIgG1
CloneP4D1
PurificationProteinGaffinitychromatography
ApplicationsWBandIF
WorkingConditionsWB(withECL):1:1000~1:3000dilution
IF:1:500IP:notrecommended
ForbestresultsofWBandIF,andotherassays(e.g.:DotandELISA),pleasedetermineoptimalworkingdilutionbytitrationtest
ReconstitutionCentrifugebrieflytocollectthepowdertothebottomofthetubepriortoreconstitution.Reconstituteeach100μgantibodyinto100μL50%glycerolinmiliQwater.Finalbuffercompositionis50mMPIPES,Ph7.0,1%sucrose,0.5%dextranand50%glycerol.Add0.02%azidetopreventbacterialcotamination.
Images(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
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ProductName:Anti-Ubiquitin(P4D1)
AlsoKnownAs:Anti-Ub
CatalogNo.:Y3012
Size:200µg
MolecularWeight:~55/25kDa
Species:Recognizesubiquitininawiderangeofspecies
Source:Immunogen:Fulllengthbovineubiquitin
Stock:lyophilizedpowder
Concentration:lyophilizedpowder
QualityAssurance:Guaranteedfordetecting5ngendogenousubiquitinorpolyubiquitinchainsinwholecellortissuelysatesatanantibodydilutionof1:1,000.
Storage:1)Storethelyophilizedantibodydesccatedat-20°C.2)Afterreconstitution,storetheantibodyat-20°C.Aliquotisnotnecessary.Thequalityoftheantibodyisguaranteedforoneyearafterpuchasingifstoredcorrectly.
PDFDataSheet:PDFDatasheet
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)

Figure1.WesternblotapplicationsAnti-Ubiquitin(P4D1)wasusedata1:500dilution.Bovinethymusubiquitinwasrunasfollows;Lane1-50ng,Lane2-25ng.Lane3-12.5ng,Lane4-6.25ng,Lane5-3.12ng.Lanes6&7represent20μgofSwiss3T3celllysatefromcellstreatedfor5hwith10µMMG-132(Lane6)oruntreatedcells(Lane7).Arrowindicatesfreeubiquitinband(8kD),highermolecularweightbandsareubiquitinatedproteins.

Figure2.IFofHeLacellsHeLacellswerestainedandvisualizedbyfluorescencemicroscopy.UbiquitintargetedcytoplasmicandnuclearproteinsandfreeubiquitinwerestainedusingAnti-Ubiquitin(P4D1)at1:500dilution.
ShippingMethod:Roomtemperatureshipping
References:N/A