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ubpbio/Epoxomicin/F1400-5/5 mg

Epoxomicin,originallyisolatedfromActinomycessp.,isacell-permeable,potent,selectiveandirreversIBLeproteasomeinhibitor.Itinhibitschymotrypsin-like,trypsin-likeandcaspase-likeactivitiesoftheproteasome.

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Epoxomicin,originallyisolatedfromActinomycessp.,isacell-permeable,potent,selectiveandirreversibleproteasomeinhibitor.Itinhibitschymotrypsin-like,trypsin-likeandcaspase-likeactivitiesoftheproteasome.

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f1400image

100nMbovine26Sproteasome(Cat.#A1200)wasincubatedwithDMSOorwith10µMEpoxomicinfor10minat37°Cin20mMTris,pH7.1,50mMNaCl,2mMATP,5mMMgCl2,2mMbMEand10%glycerol.Theproteasomewasthendiluted10Xinto50µMSUC-LLVY-AMC(Cat.#G1100)inabuffercontaining20mMTris,pH7.1,2mMbME.ThereleasedAMCfluorescencewasmonitoredbyaplatereader.

f1400image2

EqualamountofwholecelllysatespreparedfromDMSO(lane1)or20μMEpoxomicin(lane2)-treatedHeLacellswereseparatedbySDS-PAGEandimmunoblottedwithananti-Ubantibody.HEK293TCellsweretreatedwithDMSOorEpoxomicinfor4hours.

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ProductName:	Epoxomicin
AlsoKnownAs:	CAS#134381-21-8
CatalogNo.:	F1400-5
Size:	5mg
MolecularWeight:	554.72Da
Species:	N/A
Source:	Synthetic
Stock:	Powder
Concentration:	N/A
QualityAssurance:	>98%byHPLCandNMR
Storage:	Eligibleforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO.
PDFDataSheet:	DownloadPDFdatasheet
NCBIRefSeq:	N/A
Image(s):	No
ShippingMethod:	Roomtemperatureshipping
References:	1.M.Hanada,etal.(1992)J.Antibiot.,45,1746 2.L.Meng,etal.(1999)PNAS,96,10403 3.K.B.Kim,etal.Bioorg.(1999)Med.Chem.Lett.,9,3335

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ubpbio/Epoxomicin/F1400-5/5 mg

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