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ubpbio/K63Ub<sub>2</sub>/D2101/50 µg

UbchainsareformedbyconjugatingtheC-terminalglycineresidueofUbontoanyofseveninternallysineresiduesortheaminogroupofthepreviousUb.UbchainsareclassifiedbythelysineresidueusedtolinkUbs;differentUbchaintopologiescanresultindifferentsignals.Forinstance,Ubchainslinkedthroughlysine6,11,27,29,33and48arecapableoftargetingproteinsforproteasomaldegradation;incontrast,Ubchainslinkedthroughlysine63ortheN-terminalaminogroup(linearUbchains)oftenplayimportantnonproteolyticfunctionsincludingregulationofkinaseactivationandproteintranslation.AllUbchainproductsareproducedbyusingofhumanwildtypeUbreactingwithspecificE2s.

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UbchainsareformedbyconjugatingtheC-terminalglycineresidueofUbontoanyofseveninternallysineresiduesortheaminogroupofthepreviousUb.UbchainsareclassifiedbythelysineresidueusedtolinkUbs;differentUbchaintopologiescanresultindifferentsignals.Forinstance,Ubchainslinkedthroughlysine6,11,27,29,33and48arecapableoftargetingproteinsforproteasomaldegradation;incontrast,Ubchainslinkedthroughlysine63ortheN-terminalaminogroup(linearUbchains)oftenplayimportantnonproteolyticfunctionsincludingregulationofkinaseactivationandproteintranslation.AllUbchainproductsareproducedbyusingofhumanwildtypeUbreactingwithspecificE2s.
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Coomassie-stainedSDS-PAGELane1:MolecularweightMarkersLane2:5µgpurifiedK63-Ub2
Lane3:5µgpurifiedK63-Ub3Lane4:5µgpurifiedK63-Ub4
Lane5:5µgpurifiedK63-Ub5Lane6:5µgpurifiedK63-Ub6

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ProductName:K63-Ub2
AlsoKnownAs:K63-Ub2
CatalogNo.:D2101
Size:50µg
MolecularWeight:17kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheet，MSDS
NCBIRefSeq:N/A
Image(s):N/A
ShippingMethod:Dryiceshipping
References:1.HershkoA,etal.(1980)ProcNatlAcadSciUSA77(4),1783–1786.
2.PickartCM,(1997)FASEB11(13),1055–1066.3.HershkoA,etal.(1998)AnnRevBiochem67,425–479.
4.JiangX,etal.(2012)NatureReviewsImmunology12,35–48.