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ubpbio/NiIDA XPure Agarose Resin/P301025/25 ml

Nickelchelateresinsrecognizetwoexposedhistidinesforanappreciableretentionofaproteinwitha4Xor6XHistag.NickelXPureAgroseResinprovidestheadvantagesoftheNickelcationwiththehighflowratesofthehighlycross-linkedagarosebeads.Exceptforbatchpurification,thisproductisspeciallysuitableforlargescaleHis-taggedproteinpurificationusingFPLC.

TheXPureseriesofresinsaremadeforlargescaleandfinepurificationusingahigh-performanceliquidchromatography(HPLC)system.

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Nickelchelateresinsrecognizetwoexposedhistidinesforanappreciableretentionofaproteinwitha4Xor6XHistag.NickelXPureAgroseResinprovidestheadvantagesoftheNickelcationwiththehighflowratesofthehighlycross-linkedagarosebeads.Exceptforbatchpurification,thisproductisspeciallysuitableforlargescaleHis-taggedproteinpurificationusingFPLC.TheXPureseriesofresinsaremadeforlargescaleandfinepurificationusingahigh-performanceliquidchromatography(HPLC)system.

Ube2IwaspurifiedwithNi-IDAXPureAgaroseResinusingagravitycolumn
Lane1:MolecularweightMarkerLane2:Cellpelletaftersonication
Lane3:SupernatantaftersonicationLane4:Flowthroughafterbinding
Lane5:WashLane6:Elution1
Lane7:Elution2Lane8:Elution3
Lane9:Elution4Lane10:Elution5

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ProductName:Ni-IDAXPureAgaroseResin
CatalogNo.:P3010-25
Size:25ml
Bead(Geometry,size):Spherical,fine:~20-50µm
Cross-Linked:Yes
Ligand:Iminodiaceticacid(IDA)
Agarose%:6%Agarose
BindingCapacity:>26mg/mlgel(Bindingcapacitywillvaryforeachtargetprotein.)
Volume%:50%(v/v)aqueoussUSPensioncontaining20%Ethanol
StorageTemperature:2-8°C
Application:Batch,Gravity,MPLCandFPLC
PDFDataSheet:Datasheet
UsersManual:User’sGuide
Image(s):
ShippingMethod:Weticeshipping