ubpbio/Bovine immuno 20S proteasome/A1501/50 µg

UponstimulationwithIFN–γ,theexpressionofthethreecatalyticβsubunitsβ1,β2,andβ5withiso-formsβ1i(LMP2),β2i(MECL–1),andβ5i(LMP7)areinduced,respectively.Thesesubunitsareincorporatedintothe20Sproteasometoformtheimmune20Sproteasome.Itwasreportedthattheimmunoproteasomehasalteredproteolyticactivitiescomparedtoitsnormalform,whichfavorthegenerationofimmunopeptidesforantigenpresentation.

AdditionalInformation

Details

ProductName:

Bovineimmuno20Sproteasome

AlsoKnownAs:

Immuno20Sproteasome

CatalogNo.:

A1501

Size:

50µg

MolecularWeight:

700kDa

Species:

Bovine

Source:

Bovineredbloodcells

Stock:

20mMTris,20mMNaCl,1mMEDTA,5mMβME,10%Glycerol

Concentration:

Seetubelabel

QualityAssurance:

~95%bynative-PAGE,seedatasheetforgelimage

Storage:

Storeat-80°C;avoidmultiplefreeze-thawcycles

PDFDataSheet:

PDFDatasheet，MSDS

NCBIRefSeq:

N/A

Image(s):

(Clickimagetoenlarge)


Coomassie-stainedSDS-PAGE Lane1:MolecularweightMarkersLane2:5µgpurifiedBovineimmuno20Sproteasome	Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovineimmuno20Sproteasome


Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28γ(Cat.#A2300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.

ShippingMethod:

Dryiceshipping

References:

1.GoldbergAL,etal.(1992)Nature357(6377),375–379.
2.AkiM,etal.(1994)JBiochem115(2),257–269.3.TanakaK(1994)JLeukocBiol56(5),571–575.
4.ZaissDM,etal.(2011)JImmunol187(5),2302–2309.


Coomassie-stainedSDS-PAGE Lane1:MolecularweightmarkersLane2:5µgpurifiedBovineimmuno20Sproteasome	Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovineimmuno20Sproteasome


Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.	Activationof5nMimmuno20Sproteasome(Cat.#A1500)by25nMPA28γ(Cat.#A2300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively.

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ubpbio/Bovine immuno 20S proteasome/A1501/50 µg

AdditionalInformation

Details