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| ProductName: | Bovine20Sproteasome | |||||||||||||||
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| AlsoKnownAs: | 20Sproteasome | |||||||||||||||
| CatalogNo.: | A1401 | |||||||||||||||
| Size: | 50µg | |||||||||||||||
| MolecularWeight: | 700kDa | |||||||||||||||
| Species: | Bovine | |||||||||||||||
| Source: | Bovineredbloodcells | |||||||||||||||
| Stock: | 20mMTris,20mMNaCl,1mMEDTA,5mMβME,10%Glycerol | |||||||||||||||
| Concentration: | Seetubelabel | |||||||||||||||
| QualityAssurance: | ~95%bynative-PAGE,seedatasheetforgelimage | |||||||||||||||
| Storage: | Storeat-80°C;avoidmultiplefreeze-thawcycles | |||||||||||||||
| PDFDataSheet: | PDFDatasheet，MSDS | |||||||||||||||
| NCBIRefSeq: | N/A | |||||||||||||||
| Image(s): |
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| ShippingMethod: | Dryiceshipping | |||||||||||||||
| References: | 1.WaxmanL,etal.(1987)JBiolChem262(6),2451–2457. 2.GanothD,etal.(1988)JBiolChem263(25),12412–12419.3.CouxO,etal.(1996)AnnuRevBiochem65,801–847. 4.KimHM,etal.(2011)BiochimicaBiophysicaActa1809(2),67–79. |
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| Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome | Coomassie-stainednative-PAGE Lane1:5µgpurifiedBovine20Sproteasome | |
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| Activationof5nM20Sproteasomeby25nMPA700(Cat.#A1300),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. | Activationof5nM20Sproteasomeby25nMPA28α(Cat.#A2100),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. | Activationof5nM20Sproteasome(Cat.#A1400)by25nMPA28β(Cat.#A2200),theproteasomeactivitywasassayedbyusing50µMSuc-LLVY-AMC(Cat.#G1100)asthesubstrate.TheAMCfluorescencewasmonitoredbyaplatereaderwithexcitationandemissionfiltersof360±40nmand460±30nm,respectively. |