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ubpbio/6xHisUbe2B/C1200/25 µg

Ube2BisanE2enzyme,whichispartoftheE1,E2,andE3cascaderesponsibleforubiquitinationofproteinsubstrates.Itshares95%aminoacidsequenceidentitywithUbe2A.OnefunctionofUbe2BinvolvestheformationofUbe2B/Rad18(anE3enzyme)complextomonoubiquitinateproliferatingcellnuclearantigen(PCNA)thusactivatingittorepairDNAdamage.TheUbe2B/Rad18complexisrecruitedtothereplicationforkofthedamagedDNAtostartthepost-replicationrepair.

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Ube2BisanE2enzyme,whichispartoftheE1,E2,andE3cascaderesponsibleforubiquitinationofproteinsubstrates.Itshares95%aminoacidsequenceidentitywithUbe2A.OnefunctionofUbe2BinvolvestheformationofUbe2B/Rad18(anE3enzyme)complextomonoubiquitinateproliferatingcellnuclearantigen(PCNA)thusactivatingittorepairDNAdamage.TheUbe2B/Rad18complexisrecruitedtothereplicationforkofthedamagedDNAtostartthepost-replicationrepair.
Images:(Clickimagetoenlarge)
ProductName:6xHis-Ube2B
AlsoKnownAs:HR6B;UBC2;HHR6B;RAD6B;E2-17kDa
CatalogNo.:C1200
Size:25µg
MolecularWeight:17.3kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:NM_003337
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkers
Lane2:5µgpurified6xHis-Ube2B
ShippingMethod:Dryiceshipping
References:1.JentschS,etal.(1987)Nature329(6135),131–134.
2.KokenMH,etal.(1991)ProcNatlAcadSciUSA88(20),8865–8869.3.BaillyV,etal.(1994)GenesDev8(7),811–820.
4.TsujiY,etal.(2008)GenesCells13,343–354.
Coomassie-stainedSDS-PAGE
Lane1:Molecularweightmarkers
Lane2:5µgpurified6xHis-Ube2B

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