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ubpbio/(SucLeuLeuValTyr)2Rhodamine 110/G1110/2 mg
(Suc-LLVY)2-Rhodamineisafluorogenicsubstrateforthechymotrypsin–likeactivityofthe20Sand26Sproteasomes.Workingconcentrationsofthissubstrateis20-100µM.ThereleasedRhodamine110fluorescencecanbedetectedbyafluorimeterorplatereaderatexcitation/emissionof495nm/520nm,respectively.ComparedtoAMC,Rhodamine110isexcitedatlongerwavelengththatcansignificantlyenhancethesignal/noiseratio.ThissubstrateisextremlyvaluableforscreeningsmallchemicalinhibitorsofproteasomesbyavoidingtheinterferencefromchemicalcompoundautofluorescencethatoftenoccursatshorterwavelengthsaroundUVornear-UVregions.

Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicincanbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.Readingsfromproteasomeinhibitor-treatedlysatesshouldbesubtracted.

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(Suc-LLVY)2-Rhodamineisafluorogenicsubstrateforthechymotrypsin–likeactivityofthe20Sand26Sproteasomes.Theworkingconcentrationsofthissubstrateis20-100µM.ThereleasedRhodamine110fluorescencecanbedetectedbyafluorimeterorplatereaderatexcitation/emissionof495nm/520nm,respectively.ComparedtoAMC,Rhodamine110isexcitedatlongerwavelengththatcansignificantlyenhancethesignal/noiseratio.ThissubstrateisextremlyvaluableforscreeningsmallchemicalinhibitorsofproteasomesbyavoidingtheinterferencefromchemicalcompoundautofluorescencethatoftenoccursatshorterwavelengthsaroundUVornearUVregions.Whenusedtodetermineproteasomeactivityincelllysates,celllysatesthatarepre-treatedwithaproteasomeinhibitorsuchasMG132,PS341orepoxomicinshouldbeusedtodeterminethefluorescencecontributedbyothercellularproteasesthatcleavethissubstrate.Readingsfromproteasomeinhibitor-treatedlysatescanbesubtractedasbackground.

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ProductName:(Suc-Leu-Leu-Val-Tyr)2-Rhodamine110
AlsoKnownAs:(Suc-LLVY)2-Rhodamine110
CatalogNo.:G1110
Size:2mg
MolecularWeight:1507.7Da
Species:N/A
Source:Synthetic
Stock:Powder
Concentration:N/A
QualityAssurance:>98%byHPLCandNMR
Storage:Eligibleforroomtemperatureshipping.Storeat-80°Cuponreceiving;avoidmultiplefreeze-thawcyclesafterdissolvinginDMSO.
PDFDataSheet:DownloadPDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Roomtemperatureshipping
References:No