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ubpbio/6xHisUbiquitin (K0)/E1710/1 mg
Ubiquitin(Ub)isa76aminoacidproteinwidelyexpressedinthecytoplasmicandnucleusofcells.UbisposttranslationallyconjugatedtoproteinsbytheE1,E2,E3proteinubiquitinationcascade.UbcanbeconjugatedonproteinsasmonoUborpolyUbchains.Proteinubiquitinationplaysbothproteolyticandnonproteolyticfunctions.Usually,polyubiquitinatedproteinsaretargetedtothe26Sproteasomeforproteolysis.Typicalconcentrationtosupportinvitroubiquitinationis50-100µM.In6xHis-Ub(K0),alllysineresiduesweresubstitutedbyarginines,therebyitcanonlysupportmonoubiquitination,butnotpolyubiquitinchains.

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Ubiquitin(Ub)isa76aminoacidproteinwidelyexpressedinthecytoplasmicandnucleusofcells.UbisposttranslationallyconjugatedtoproteinsbytheE1,E2,E3proteinubiquitinationcascade.UbcanbeconjugatedonproteinsasmonoUborpolyUbchains.Proteinubiquitinationplaysbothproteolyticandnonproteolyticfunctions.Usually,polyubiquitinatedproteinsaretargetedtothe26Sproteasomeforproteolysis.Typicalconcentrationtosupportinvitroubiquitinationis50-100µM.In6xHis-Ub(K0),alllysineresiduesweresubstitutedbyarginines,therebyitcanonlysupportmonoubiquitination,butnotpolyubiquitinchains.
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Coomassie-stainedSDS-PAGELane1:MolecularweightMarkersLane2:5µgpurified6xHis-Ubiquitin(K0)
Lane3:5µgpurified6xHis-Ubiquitin(K6R)Lane4:5µgpurified6xHis-Ubiquitin(K11R)
Lane5:5µgpurified6xHis-Ubiquitin(K27R)Lane6:5µgpurified6xHis-Ubiquitin(K29R)
Lane7:5µgpurified6xHis-Ubiquitin(K33R)Lane8:5µgpurified6xHis-Ubiquitin(K48R)
Lane9:5µgpurified6xHis-Ubiquitin(K63R)

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ProductName:6xHis-Ubiquitin(K0)
AlsoKnownAs:6xHis-UbK0
CatalogNo.:E1710
Size:1mg
MolecularWeight:8.5kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bySDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheet，MSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Dryiceshipping
References:1.HershkoA,etal.(1980)ProcNatlAcadSciUSA77(4),1783–1786.
2.PickartCM(1997)FASEB11(13),1055–1066.3.HershkoA,etal.(1998)AnnRevBiochem67,425–479.
4.JiangX,etal.(2012)NatureReviewsImmunology12,35–48.5.BremmA,etal.(2012)MethodsMolBiol832,291–228.