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ubpbio/6xHisUbe2E2 (UbcH8)/C1900/25 µg

Ube2E2isanE2enzyme,whichispartoftheE1,E2,andE3cascaderesponsibleforubiquitinationofproteinsubstrates.Itshares96%identicalaminoacidsequencewithUbe2E1andUbe2E3withtheexceptionoftheN-terminalextensionthathaslittleincommonwiththeothertwoE2s.Ube2E2interactswithARA54andRNF8.TheRNF8/Ube2E2interactionisfoundtocatalyzeK48-linkedpoly-ubiquitination.Ube2E2mayplayaroleinubiquitinationandsubsequentproteasomaldegradationoftheE3UbligaseParkin.

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Ube2E2isanE2enzyme,whichispartoftheE1,E2,andE3cascaderesponsibleforubiquitinationofproteinsubstrates.Itshares96%identicalaminoacidsequencewithUbe2E1andUbe2E3withtheexceptionoftheN-terminalextensionthathaslittleincommonwiththeothertwoE2s.Ube2E2interactswithARA54andRNF8.TheRNF8/Ube2E2interactionisfoundtocatalyzeK48-linkedpoly-ubiquitination.Ube2E2mayplayaroleinubiquitinationandsubsequentproteasomaldegradationoftheE3UbligaseParkin.
Images:(Clickimagetoenlarge)
ProductName:6xHis-Ube2E2(UbcH8)
AlsoKnownAs:UBCH8
CatalogNo.:C1900
Size:25µg
MolecularWeight:22.3kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~90%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:NM_152653
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkers
Lane2:5µgpurified6xHis-Ube2E2(UbcH8)
ShippingMethod:Dryiceshipping
References:1.KimuraM,etal.(1997)CytogenetCellGenet78(2),107–111.
2.ZhangY,etal.(2000)ProcNatlAcadSciUSA97(24),13354–13359.3.ItoK,etal.(2001)EuropeanJournalofBiochemistry268,2725–2732.
4.LokGTM,etal.(2012)NucleicAcidsRes40(1),196-205.
Coomassie-stainedSDS-PAGE
Lane1:Molecularweightmarkers
Lane2:5µgpurified6xHis-Ube2E2(UbcH8)

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