Deprecated: Required parameter $cat_id follows optional parameter $type in /www/wwwroot/ebimall.com/systems/hong.php on line 2088

Deprecated: Required parameter $where follows optional parameter $tree_id in /www/wwwroot/ebimall.com/systems/hlb.php on line 3505
ubpbio/IPTG/P1010500/5 X 100 g188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
您好,欢迎您进入188进口试剂采购网网站! 服务热线:4000-520-616
蚂蚁淘商城 | 现货促销 | 科研狗 | 生物在线

ubpbio/IPTG/P1010500/5 X 100 g
IPTG,Isopropylβ-D-1-thiogalactopyranoside,bindsthelacrepressorandinducesexpressionofβ-galactosidaseinE.coli.IPTGiswidelyusedtoinducetheexpressionofclonedgenesunderthecontrolofthelacoperonandtoscreenblue/whitecolonyinX-galplate.

Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.

AdditionalInformation

Details

IPTG,Isopropylβ-D-1-thiogalactopyranoside,bindsthelacrepressorandinducesexpressionofβ-galactosidaseinE.coli.IPTGiswidelyusedtoinducetheexpressionofclonedgenesunderthecontrolofthelacoperonandtoscreenblue/whitecolonyinX-galplate.
Note:Toprepare10mL1MIPTGstocksolution,weight2.38gIPTGanddissolvein8mldistilledwater,adjusttoafinalvolumeof10mlwithdistilledwater.Sterilethesolutionusinga0.22umsyringefilter.Aliquotthestockandstoreunder-20°Cforuptooneyear.
ExpressionofGST-UCHL3inBL21(DE3)cellswasinducedbytheadditionof25μM(lane3),100μM(lane4),200μM(lane5)or400μM(lane6)IPTGat370Cfor2hours.Lane2withoutIPTG.

新闻动态
行业前沿
技术文章
最新产品
ProductName:IPTG
AlsoKnownAs:isopropyl-β-D-thiogalactopyranoside
CatalogNo.:P1010-500
Size:5X100g
CAS:367-93-1
Formula:C9H18O5S
MolecularWeight:238.3
Form:Powder
QualityAssurance:>99%byHPLC,dioxane-free
Source:Synthetic
Storage:Eligibleforambienttemperatureshipping.Storeat4°Cuponreceiving.
PDFDataSheet:PDFDatasheet
Image(s):
ShippingMethod:Roomtemperatureshipping
References:J.Sambrook,D.W.Russell,MolecularCloning:ALaboratoryManual,theThirdedition(ColdSpringHarborLaboratoryPress,ColdSpringHarbor,NewYork,A1.27,2001)pp.1.124-1.125.