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ubpbio/6xHisUbiquitin<sub>(175)</sub>/E1310/1 mg

Ub75lackstheaminoacidglycine76,whichisrequiredtoformthioesterbondwithUbactivatingenzymeE1.Therefore,Ub75cannotreactwithE1,E2andE3enzymes;anddoesnotsupportubiquitination.Itcanbeusedasanegativecontrolinubiquitinationreactions;ortoassaybindingofUbwithUbbindingproteinsorubiquitinationenzymes.

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Ub75lackstheaminoacidglycine76,whichisrequiredtoformthioesterbondwithUbactivatingenzymeE1.Therefore,Ub75cannotreactwithE1,E2andE3enzymes;anddoesnotsupportubiquitination.Itcanbeusedasanegativecontrolinubiquitinationreactions;ortoassaybindingofUbwithUbbindingproteinsorubiquitinationenzymes.
Images:(Clickimagetoenlarge)
ProductName:6xHis-Ubiquitin(1-75)
AlsoKnownAs:6xHis-Ub(1-75)
CatalogNo.:E1310
Size:1mg
MolecularWeight:8.5kDa
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:N/A
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkers
Lane2:5µgpurifiedUbiquitin(1-75)
Lane3:5µgpurified6xHis-Ubiquitin(1-75)
ShippingMethod:Dryiceshipping
References:1.HershkoA,etal.(1980)ProcNatlAcadSciUSA77(4),1783–1786.
2.PickartCM(1997)FASEB11(13),1055–1066.3.HershkoA,etal.(1998)AnnRevBiochem67,425–479.
4.JiangX,etal.(2012)NatureReviewsImmunology12,35–48.5.BremmA,etal.(2012)MethodsMolBiol832,291–228.
Coomassie-stainedSDS-PAGE
Lane1:Molecularweightmarkers
Lane2:5µgpurifiedUbiquitin(1-75)
Lane3:5µgpurified6xHis-Ubiquitin(1-75)

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