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ubpbio/6xHisSAE1/SAE2/B1300/25 µg

SAE1/SAE2istheheterodimericE1enzymethatconjugatesSUMOtoproteinsubstrates.Theconjugationprocessisverysimilartothatofproteinubiquitination.SUMOisconjugatedontothecatalyticcysteineresidueofSAE2byformationofathioesterbondinanATP-dependentreaction.TheactivatedSUMOisthenpassedontoanE2(Ubc9)tobefinallyattachedtoasubstrate.

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SAE1/SAE2istheheterodimericE1enzymethatconjugatesSUMOtoproteinsubstrates.Theconjugationprocessisverysimilartothatofproteinubiquitination.SUMOisconjugatedontothecatalyticcysteineresidueofSAE2byformationofathioesterbondinanATP-dependentreaction.TheactivatedSUMOisthenpassedontoanE2(Ubc9)tobefinallyattachedtoasubstrate.
Images:(Clickimagetoenlarge)
ProductName:6xHis-SAE1/SAE2
AlsoKnownAs:SAE1(AOS1;SUA1;UBLE1A;HSPC140;FLJ3091);SAE2(RBBP8;RIM;CTIP;JWDS;SCKL2)
CatalogNo.:B1300
Size:25µg
MolecularWeight:SAE1(33kDa),SAE2(71.2kDa)
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:~95%bySDS-PAGE,seedatasheetforgelimage
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFDatasheetMSDS
NCBIRefSeq:NM_005500(SAE1);NM_002894(SAE2)
Image(s):(Clickimagetoenlarge)
Coomassie-stainedSDS-PAGE
Lane1:MolecularweightMarkers
Lane2:5µgpurified6xHis-SAE1/SAE2
ShippingMethod:Dryiceshipping
References:1.DesterroJM,etal.(1999)JBiolChem274(15),10618–10624.
2.MelchiorF(2000)AnnuRevCellDevBiol16,591–626.3.SchulmanBA,etal.(2009)NatRevMolBiol10(5),319–331.
Coomassie-stainedSDS-PAGE
Lane1:Molecularweightmarkers
Lane2:5µgpurified6xHis-SAE1/SAE2

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