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ubpbio/Sumo1, Sumo2, Sumo 3 Conjugation Kit/J3140/1 Kit
Sumo1,Sumo2,Sumo3ConjugationKitisdesignedforassayingtheconjugationofSumo1,Sumo2andSumo3toproteinsubstratesinvitro.Inaproteinsumoylationreaction,aSumoproteinisfirstactivatedbytheSumoactivatingenzymeSAE1/SAE2(E1)inanATPhydrolysis-dependentmanner;activatedSumoisthentransferredtotheSumoconjugatingenzymeUbe2I(E2)byformationofathiolesterbondwiththecatalyticcysteineresidueofUbe2I;Sumo-chargedUbe2IcanmediateproteinsumoylationinthepresenceofanappropriateSumoligase(E3).

AdditionalInformation

Details

*Thiskitissufficientforsettingup25x20µlreactions.
Description
Sumo1,Sumo2,Sumo3ConjugationKitisdesignedforassayingtheconjugationofSumo1,Sumo2andSumo3toproteinsubstratesinvitro.Inaproteinsumoylationreaction,aSumoproteinisfirstactivatedbytheSumoactivatingenzymeSAE1/SAE2(E1)inanATPhydrolysis-dependentmanner;activatedSumoisthentransferredtotheSumoconjugatingenzymeUbe2I(E2)byformationofathiolesterbondwiththecatalyticcysteineresidueofUbe2I;Sumo-chargedUbe2IcanmediateproteinsumoylationinthepresenceofanappropriateSumoligase(E3).
Atypical20µlreactioncontains100nMSAE1/SAE2,2µM6xHis-Ube2I,50µMSumo1(orSumo2,orSumo3),4mMATP,2µME3(notsupplied)and2µMproteinsubstrate(nosupplied)inthesumoylationbuffer.
Components
•20XSAE1/SAE2(2µM).........................25µl•20X6xHis-Ube2I(40µM)........................25µl
•10XSumo1(500µM)..............................50µl•10XSumo2(500µM)..............................50µl
•10XSumo3(500µM)..............................50µl•10XATP(40mM).................................125µl
•10XSumoylationBuffer...........................1ml

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ProductName:Sumo1,Sumo2,Sumo3ConjugationKit
AlsoKnownAs:N/A
CatalogNo.:J3140
Size:1Kit
MolecularWeight:N/A
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:95%basedonSDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):N/A
ShippingMethod:Dryiceshipping
References:N/A