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ubpbio/Nedd8 Conjugation Kit/J3150/1 Kit
Nedd8ConjugationKitisdesignedforassayingtheconjugationofNedd8toproteinsubstratesinvitro.Inaproteinneddylationreaction,Nedd8isfirstactivatedbytheNedd8activatingenzymeAPPBP1/UBA3(E1)inanATPhydrolysis-dependentmanner;activatedNedd8isthentransferredtotheNedd8conjugatingenzymeUbe2M(UbcH12)(E2)byformationofathiolesterbondwiththecatalyticcysteineresidueofUbcH12I;Nedd8-chargedUbe2M(UbcH12)canmediateproteinneddylationinthepresenceofanappropriateNedd8ligase(E3).

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Details

*Thiskitissufficientforsettingup25x20µlreactions.
Description
Nedd8ConjugationKitisdesignedforassayingtheconjugationofNedd8toproteinsubstratesinvitro.Inaproteinneddylationreaction,Nedd8isfirstactivatedbytheNedd8activatingenzymeAPPBP1/UBA3(E1)inanATPhydrolysis-dependentmanner;activatedNedd8isthentransferredtotheNedd8conjugatingenzymeUbe2M(UbcH12)(E2)byformationofathiolesterbondwiththecatalyticcysteineresidueofUbcH12I;Nedd8-chargedUbe2M(UbcH12)canmediateproteinneddylationinthepresenceofanappropriateNedd8ligase(E3).
Atypical20µlreactioncontains100nMAPPBP1/6xHis-UBAs,2µM6xHis-Ube2M(UbcH12),15µM6xHis-Nedd8,4mMATP,2µME3(notsupplied)and2µMproteinsubstrate(nosupplied)intheNeddylationBuffer.
Components
•20XAPPBP1/6xHis-UBA3(2µM)................25µl•20X6xHis-Ube2M(UbcH12)(40µM))..........25µl
•10X6xHis-Nedd8(150µM)..........................50µl•10XATP(40mM)........................................60µl
•10XNeddylationBuffer...............................100µl

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ProductName:Nedd8ConjugationKit
AlsoKnownAs:N/A
CatalogNo.:J3150
Size:1Kit
MolecularWeight:N/A
Species:Human
Source:Bacterialrecombinant
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:95%basedonSDS-PAGE
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Dryiceshipping
References:N/A