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ubpbio/NiNTA XPure Agarose Resin/P3020100/100 ml
Ni-NTAXPureAgaroseResincanbeusedtopurify6xHis-taggedproteinsexpressedinseriesofexpressionvectors,suchasE.coli.,yeast,insectcellsandmammaliancells.Ni-NTAXPureAgaroseResinconsistsof90µmbeadsofhighlycross-linked6%agarose,towhichNitilotriaceticacid(NTA)hasbeencoupled.Thechelatinggrouphasthenbeenchargedwithnickelions(Ni2+).Thisformisverystableoctahedralstructureofnickelionsinthecenter,whichcanprotectthenickelionsfromattackofthecompetitivesmallmolecule.ThestructureofNi-NTAiscompatiblewithacertainconcentrationofreducingagents,denaturingagents,detergentsandotheradditives.Ni-NTAXPureAgaroseResinishighlystableandexpandtherangeofsuitableoperatingconditions.

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Ni-NTAXPureAgaroseResincanbeusedtopurify6xHis-taggedproteinsexpressedinseriesofexpressionvectors,suchasE.coli.,yeast,insectcellsandmammaliancells.Ni-NTAXPureAgaroseResinconsistsof90µmbeadsofhighlycross-linked6%agarose,towhichNitilotriaceticacid(NTA)hasbeencoupled.Thechelatinggrouphasthenbeenchargedwithnickelions(Ni2+).Thisformisverystableoctahedralstructureofnickelionsinthecenter,whichcanprotectthenickelionsfromattackofthecompetitivesmallmolecule.ThestructureofNi-NTAiscompatiblewithacertainconcentrationofreducingagents,denaturingagents,detergentsandotheradditives.Ni-NTAXPureAgaroseResinishighlystableandexpandtherangeofsuitableoperatingconditions.

TheXPureseriesofresinsaremadeforlargescaleandfinepurificationusingahigh-performaceliquidchromatography(HPLC)system.

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ProductName:Ni-NTAXPureAgaroseResin
CatalogNo.:P3020-100
Size:100ml
Bead(Geometry,size):45-165µm
Cross-Linked:Yes
Ligand:Nitilotriaceticacid(NTA)
Agarose%:Highlycross-linked6%agarose
BindingCapacity:>40mg6xHis-taggedprotein/mlmedium
Volume%:50%(v/v)aqueoussuspensioncontaining20%Ethanol
StorageTemperature:2-8°C
Application:Batch,Gravity,MPLCandFPLC
PDFDataSheet:PDFDatasheet
UsersManual:PDFUser’sGuide
Image(s):No
ShippingMethod:Weticeshipping