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ubpbio/Proteasome Activity Fluorometric Assay Kit I/J4110/1 Kit
ProteasomeActivityFluorometricAssayKitIisdesignedforassayingtheproteasome’schymotrypsin-likeactivityinvitrousingpurifiedproteasome,clearedcelllysatesortissueextracts.TheproteasomecleavesSUC-LLVY-AMCandthereleasedAMCfluorescencecanbemonitoredusingaplatereaderorfluorometerattheexcitation/emissionwavelengthof360nm/460nm,respectively.

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*ThesuppliedSuc-LLVY-AMCand20Sproteasomearesufficientfor165Xand10X150µlreactions,respectively.
Description
ProteasomeActivityFluorometricAssayKitIisdesignedforassayingtheproteasome’schymotrypsin-likeactivityinvitrousingpurifiedproteasome,clearedcelllysatesortissueextracts.TheproteasomecleavesSUC-LLVY-AMCandthereleasedAMCfluorescencecanbemonitoredusingaplatereaderorfluorometerattheexcitation/emissionwavelengthof360nm/460nm,respectively.Theamountofthecelllysatesortissueextractstobeusedinthereactionshouldbeoptimized.
AMCisusedtoproduceastandardcurveusingaconcentrationrangeof10-100pmol.
Dissolve1mgMG132in105µlDMSOtomakea20mMstocksolutionbeforeuse,using50-200µMMG132toinhibittheproteasomeinvitro.
Thebovine20Sproteasomecanbeusedasapositivecontrol.Atypical150µlreactioncontains10nM20Sproteasome,50µMfluorogenicsubstrateintheassaybuffer.
Components
•AMC(0.1mMinDMSO)................................................30µl•1000XSUC-LLVY-AMC(50mMinDMSO)...................25µl
•40XBovine20SProteasome(400nM).........................40µl•MG-132..........................................................................1mg
•DMSO..........................................................................200µl•20XProteasomeAssayBuffer....................................1.35ml

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ProductName:ProteasomeActivityFluorometricAssayKitI
AlsoKnownAs:N/A
CatalogNo.:J4110
Size:1Kit
MolecularWeight:N/A
Species:N/A
Source:N/A
Stock:20mMTris,150mMNaCl,2mMβME,10%Glycerol
Concentration:Seetubelabel
QualityAssurance:Enzyme>95%bySDS-PAGE;Inhibitorandsubstrate>98%byHPLCandNMR
Storage:Storeat-80°C;avoidmultiplefreeze-thawcycles
PDFDataSheet:PDFdatasheet,MSDS
NCBIRefSeq:N/A
Image(s):No
ShippingMethod:Dryiceshipping
References:N/A