AntiIRS1 Antibody (PhosphoSer 312) (OAAI00339)

货号：	OAAI00339
抗体英文名：	Anti-IRS-1Antibody(Phospho-Ser312)(OAAI00339)
靶点：	IRS1
应用范围：	WB,IP
宿主：	Rabbit
适应物种：	Human,Rat,Mouse
克隆性：	否
保存条件：	Theantibodiesarestablefor12monthsfromdateofreceiptwhenstoredat-20Cto-70C.Theantibodiescanbestoredat2Cto8Cforthreemonthwithoutdetectablelossofactivity. Avoidrepeatedfreezingthawingcycles.
亚型：	RabbitIgG
免疫原：	AsyntheticpeptidecorrespondingtotheepitopeATSPAwithasinglephosphorylationsiteSer312ofHumanIRS-1.
规格：	100 ug

NCBIGeneId:3667
SwissprotId:P35568
InsulinReceptorSubstRate-1(IRS-1),165kDacytoplasmicdockingprotein,isoneofthemajorendogenoussubstRatesoftheinsulinreceptorkinase.IRS-1containsmultipletyrosinephosphorylationmotifsthatserveasdockingsitesforSH2domaincontainingproteins,whichmediatethemetabolicandgrowthpromotingfunctionsofinsulin.IRS-1alsocontainsover30potentialserine/threoninephosphorylationsites.Ser312ofIRS-1isphosphorylatedbyJNKandIKKandSer789isphosphorylatedbySIK-2,amemberofAMPKfamily.ThephosphorylationofTyr612andSer636/639ismediatedbythePKCandmTORpathways,respectivelyandphosphorylationatSer1101ismediatedbyPKC,resultinginaninhibitionofinsulinsignalinginthecell,sμggestingapotentialmechanismforinsulinresistanceinsomemodelsofobesity.-Seemoreat:http://www.avivasysbio.com/sd/tds/html_datasheet.php?sku=OAAI00339

Phospho-peptide,non-phospho-peptide

DotBlot:1?gpeptidewasblotontoNCmembraneA:IRS-1(pS312)B:IRS-1(nonphosphorylated)C:Non-relatedPhosphopeptide)Followedbyrabbitantibodiesincubationata1:1000dilution:1:Rabbitanti-IRS-1(pS312)(Cat#OAAI00339)2:Rabbitanti-IRS-1(PairedS312)Cat#(602-620)

insulinstimulatedCHO

WesternBlot:ThecelllysatederivedfrominsulinstimulatedCHOwasimmunoprecipitatedbyRabbitantiIRS-1(pS312),(Cat#OAAI00339),thenimmunoprobedbythesameantibodyat1:500(lane1).Lane2isanegativecontrol.