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avivasysbio/Recombinant Human sAPO-1/Fas Protein (OPTA00867)/OPTA00867

TestedSpeciesReactivity:
Human
ProductFormat:
Lyophilized
Application:
CA
AdditionalInformation:
Description:Fas(APO-1,CD95,TNFRSF6)isamemberoftheTNFreceptorsuperfamilyandthroughalternativesplicing,existsinmultipleisoformsincludingasolubleform.BindingofFastoitsreceptor,Fasligand(FasL),triggersapoptosisthroughtherecruitmentofFADDandactivationofthecaspasecascade.sFasiscapableofinhibitingFasL-inducedapoptosisbyactingasadecoyreceptorthatservesasasinkforFasL.FasexpressionhasbeendetectedinavarietyofcelltypesincludingactivatedTandBcells,fibroblasts,dendriticcells,thymocytes,macrophages,hepatocytes,cardiomyocytesandmalignanthumanlymphoidcelllines.
:::
Bioactivity:TheED50wasdeterminedbyitsABIlitytoinhibitthecytotoxicityofJurkatcellsisbetween10-15ug/mlinthepresenceof2ng/mlofhFasL.
:::
EndotoxinLevel:Endotoxinlevelis<0.1 ng/ug="" of="" protein=""><1>
ReconstitutionandStorage:
-20C
Purity:
98%
ProteinSequence:
MRLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRS
Source:
E.coli
TargetReference:

PapoffG,CascinoI,EramoA,StaraceG,LynchDHandRubertiG.1996.JImmunol.156(12):4622-4630.

ThorburnA.2004.CellSignal.16(2):139-144.

StrasserA,JostPJandNagataS.2009.Immunity.30(2):180-192.

BouilletPandO"™ReillyLA.2009.NatRevImmunol.9(7):514-519.

Datasheets/Manuals:
PrintabledatasheetforOPTA00867
AliasSymbols:
solubleFasreceptor(sFasR),TNFRSF6,CD95,ApoI,FasAntigen
MolecularWeight:
17.6kDa
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